Boulberdaa, M. et al. (2016) A role for the long non-coding RNA SENCR in commitment and function of endothelial cells. Molecular Therapy, 24(5), pp. 978-990. (doi: 10.1038/mt.2016.41) (PMID:26898221) (PMCID:PMC4876031)
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Abstract
Despite the increasing importance of long non-coding RNA in physiology and disease, their role in endothelial biology remains poorly understood. Growing evidence has highlighted them to be essential regulators of human embryonic stem cell differentiation. SENCR, a vascular-enriched long non-coding RNA, overlaps the Friend Leukemia Integration virus 1 (FLI1) gene, a regulator of endothelial development. Therefore, we wanted to test the hypothesis that SENCR may contribute to mesodermal and endothelial commitment as well as in endothelial function. We thus developed new differentiation protocols allowing generation of endothelial cells from human embryonic stem cells using both directed and haemogenic routes. The expression of SENCR was markedly regulated during endothelial commitment using both protocols. SENCR did not control the pluripotency of pluripotent cells; however its overexpression significantly potentiated early mesodermal and endothelial commitment. In HUVEC, SENCR induced proliferation, migration and angiogenesis. SENCR expression was altered in vascular tissue and cells derived from patients with critical limb ischemia and premature coronary artery disease compared to controls. Here, we showed that SENCR contributes to the regulation of endothelial differentiation from pluripotent cells and controls the angiogenic capacity of HUVEC. These data give novel insight into the regulatory processes involved in endothelial development and function.
| Item Type: | Articles |
|---|---|
| Additional Information: | Further funding from British Heart Foundation Centre for Vascular Reparation grant (RM/13/1/30158) to University of Edinburgh. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Baker, Professor Andrew and Garcia, Dr Raquel and Mountford, Dr Joanne and McBride, Dr Martin and Boulberdaa, Dr Mounia and McClure, Dr John |
| Authors: | Boulberdaa, M., Scott, E., Ballantyne, M., Garcia, R., Descamps, B., Angelini, G. D., Brittan, M., Hunter, A., McBride, M., McClure, J., Miano, J. M., Emanueli, C., Mills, N. L., Mountford, J. C., and Baker, A. H. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | Molecular Therapy |
| Publisher: | Nature Publishing Group |
| ISSN: | 1525-0016 |
| ISSN (Online): | 1525-0024 |
| Copyright Holders: | Copyright © 2016 The American Society of Gene & Cell Therapy |
| First Published: | First published in Molecular Therapy 24(5): 978-990 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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