Efficient generation of vesicular stomatitis 1 virus (VSV)-pseudotypes bearing morbilliviral glycoproteins and their use in quantifying virus neutralising antibodies

Logan, N. et al. (2016) Efficient generation of vesicular stomatitis 1 virus (VSV)-pseudotypes bearing morbilliviral glycoproteins and their use in quantifying virus neutralising antibodies. Vaccine, 34(6), pp. 814-822. (doi:10.1016/j.vaccine.2015.12.006) (PMID:6706278)

[img]
Preview
Text
113593.pdf - Published Version
Available under License Creative Commons Attribution.

1MB

Abstract

Morbillivirus neutralising antibodies are traditionally measured using either plaque reduction neutralisation tests (PRNTs) or live virus microneutralisation tests (micro-NTs). While both test formats provide a reliable assessment of the strength and specificity of the humoral response, they are restricted by the limited number of viral strains that can be studied and often present significant biological safety concerns to the operator. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSVΔG) based pseudotyping system for the measurement of morbillivirus neutralising antibodies. By expressing the haemagglutinin (H) and fusion (F) proteins of canine distemper virus (CDV) on VSVΔG pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Further, by exchanging the glycoprotein expression construct, responses against distinct viral strains or species may be measured. Using this technique, we demonstrate cross neutralisation between CDV and peste des petits ruminants virus (PPRV). As an example of the value of the technique, we demonstrate that UK dogs vary in the breadth of immunity induced by CDV vaccination; in some dogs the neutralising response is CDV-specific while, in others, the neutralising response extends to the ruminant morbillivirus PPRV. This technique will facilitate a comprehensive comparison of cross-neutralisation to be conducted across the morbilliviruses.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Haydon, Professor Daniel and Baron, Dr Michael and Willett, Professor Brian and McDonald, Mr Michael and Cleaveland, Professor Sarah and McMonagle, Mrs Elizabeth and Logan, Miss Nicola and Takahashi, Ms Emi
Authors: Logan, N., McMonagle, E., Drew, A. A., Takahashi, E., McDonald, M., Baron, M., Gilbert, M., Cleaveland, S., Haydon, D. T., Hosie, M. J., and Willett, B.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Vaccine
Publisher:Elsevier B.V.
ISSN:0264-410X
ISSN (Online):1873-2518
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Vaccine 34(6):814-822
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record