Borax-Loaded PLLA for promotion of myogenic differentiation

Rico Tortosa, P., Rodrigo-Navarro, A. and Salmeron-Sanchez, M. (2015) Borax-Loaded PLLA for promotion of myogenic differentiation. Tissue Engineering Part A, 21(21-22), pp. 2662-2672. (doi:10.1089/ten.tea.2015.0044) (PMID:26239605)

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Abstract

Boron is an essential metalloid, which plays a key role in plant and animal metabolisms. It has been reported that boron is involved in bone mineralization, has some uses in synthetic chemistry, and its potential has been only recently exploited in medicinal chemistry. However, in the area of tissue engineering, the use of boron is limited to works involving certain bioactive glasses. In this study, we engineer poly(l-lactic acid) (PLLA) substrates with sustained release of boron. Then, we analyze for the first time the uniqueness effects of boron in cell differentiation using murine C2C12 myoblasts and discuss a potential mechanism of action in cooperation with Ca2+. Our results demonstrate that borax-loaded materials strongly enhance myotube formation at initial steps of myogenesis. Furthermore, we demonstrate that Ca2+ plays an essential role in combination with borax as chelating or blocking Ca2+ entry into the cell leads to a detrimental effect on myoblast differentiation observed on borax-loaded materials. This research identifies borax-loaded materials to trigger differentiation mechanisms and it establishes a new tool to engineer microenvironments with applications in regenerative medicine for muscular diseases.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Rodrigo-Navarro, Mr Aleixandre and Rico Tortosa, Dr Patricia
Authors: Rico Tortosa, P., Rodrigo-Navarro, A., and Salmeron-Sanchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Tissue Engineering Part A
Publisher:Mary Ann Liebert
ISSN:1937-3341
ISSN (Online):1937-335X
Copyright Holders:Copyright © Mary Ann Liebert
First Published:First published in Tissue Engineering Part A 21(21-22):2662-2672

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING