Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays

MacDonald, N. P., Feng, Z., Hall, C., Reboud, J. , Crosier, K., Crosier, P., Patton, E., Wlodkowic, D. and Cooper, J. M. (2016) Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays. Lab on a Chip, 2016(16), p. 297. (doi:10.1039/C5LC01374G) (PMID:26646354)

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Abstract

3D printing has emerged as a rapid and cost-efficient manufacturing technique to enable the fabrication of bespoke, complex prototypes. If the technology is to have a significant impact in biomedical applications, such as drug discovery and molecular diagnostics, the devices produced must be biologically compatible to enable their use with established reference assays and protocols. In this work we demonstrate that we can adapt the Fish Embryo Test (FET) as a new method to quantify the toxicity of 3D printed microfluidic devices. We assessed the biocompatibility of four commercially available 3D printeing polymers (VisiJetCrystal EX200, Watershed 11122XC, Fototec SLA 7150 Clear and ABSplus P-430), through the observation of key developmental markers in the developing zebrafish embryos. Results show all of the materials to be highly toxic to the embryos, resulting in fatality, although we do demonstrate that post-printing treatment of Fototec 7150 makes it suitable for zebrafish culture within the FET.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wlodkowic, Dr Donald and Cooper, Professor Jonathan and Reboud, Dr Julien
Authors: MacDonald, N. P., Feng, Z., Hall, C., Reboud, J., Crosier, K., Crosier, P., Patton, E., Wlodkowic, D., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Copyright Holders:Copyright © 2016 The Royal Society of Chemistry
First Published:First published in Lab on a Chip 2016(16):291-297
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.238

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING