Turnover rate of NS3 proteins modulates bluetongue virus replication kinetics in a host-specific manner

Ftaich, N. et al. (2015) Turnover rate of NS3 proteins modulates bluetongue virus replication kinetics in a host-specific manner. Journal of Virology, 89(20), pp. 10467-10481. (doi: 10.1128/jvi.01541-15) (PMID:26246581)

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Bluetongue virus (BTV) is an arbovirus transmitted to livestock by midges of the Culicoides family and is the etiological agent of a hemorrhagic disease in sheep and other ruminants. In mammalian cells, BTV particles are released primarily by virus-induced cell lysis, while in insect cells they bud from the plasma membrane and establish a persistent infection. BTV possesses a ten-segmented double-stranded RNA genome, and NS3 proteins are encoded by segment 10 (Seg-10). The viral nonstructural protein 3 (NS3) plays a key role in mediating BTV egress as well as in impeding the in vitro synthesis of type I interferon in mammalian cells. In this study, we asked whether genetically distant NS3 proteins can alter BTV-host interactions. Using a reverse genetics approach, we showed that, depending on the NS3 considered, BTV replication kinetics varied in mammals but not in insects. In particular, one of the NS3 proteins analyzed harbored a proline at position 24 that leads to its rapid intracellular decay in ovine but not in Culicoides cells and to the attenuation of BTV virulence in a mouse model of disease. Overall, our data reveal that the genetic variability of Seg-10/NS3 differentially modulates BTV replication kinetics in a host-specific manner and highlight the role of the host-specific variation in NS3 protein turnover rate.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Ratinier, Dr Maxime and Palmarini, Professor Massimo and Barry, Dr Gerald
Authors: Ftaich, N., Ciancia, C., Viarouge, C., Barry, G., Ratinier, M., van Rijn, P. A., Breard, E., Vitour, D., Zientara, S., Palmarini, M., Terzian, C., Arnaud, F., and López, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virology
Publisher:American Society for Microbiology
ISSN (Online):1098-5514

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