The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes

Bishop, R.P., Hemmink, J.D., Morrison, W.I., Weir, W. , Toye, P.G., Sitt, T., Spooner, P.R., Musoke, A.J., Skilton, R.A. and Odongo, D.O. (2015) The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes. International Journal for Parasitology: Parasites and Wildlife, 4(3), pp. 333-342. (doi:10.1016/j.ijppaw.2015.08.006) (PMID:26543804) (PMCID:PMC4589832)

Bishop, R.P., Hemmink, J.D., Morrison, W.I., Weir, W. , Toye, P.G., Sitt, T., Spooner, P.R., Musoke, A.J., Skilton, R.A. and Odongo, D.O. (2015) The African buffalo parasite Theileria. sp. (buffalo) can infect and immortalize cattle leukocytes and encodes divergent orthologues of Theileria parva antigen genes. International Journal for Parasitology: Parasites and Wildlife, 4(3), pp. 333-342. (doi:10.1016/j.ijppaw.2015.08.006) (PMID:26543804) (PMCID:PMC4589832)

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Abstract

African Cape buffalo (Syncerus caffer) is the wildlife reservoir of multiple species within the apicomplexan protozoan genus Theileria, including Theileria parva which causes East coast fever in cattle. A parasite, which has not yet been formally named, known as Theileria sp. (buffalo) has been recognized as a potentially distinct species based on rDNA sequence, since 1993. We demonstrate using reverse line blot (RLB) and sequencing of 18S rDNA genes, that in an area where buffalo and cattle co-graze and there is a heavy tick challenge, T. sp. (buffalo) can frequently be isolated in culture from cattle leukocytes. We also show that T. sp. (buffalo), which is genetically very closely related to T. parva, according to 18s rDNA sequence, has a conserved orthologue of the polymorphic immunodominant molecule (PIM) that forms the basis of the diagnostic ELISA used for T. parva serological detection. Closely related orthologues of several CD8 T cell target antigen genes are also shared with T. parva. By contrast, orthologues of the T. parva p104 and the p67 sporozoite surface antigens could not be amplified by PCR from T. sp. (buffalo), using conserved primers designed from the corresponding T. parva sequences. Collectively the data re-emphasise doubts regarding the value of rDNA sequence data alone for defining apicomplexan species in the absence of additional data. ‘Deep 454 pyrosequencing’ of DNA from two Theileria sporozoite stabilates prepared from Rhipicephalus appendiculatus ticks fed on buffalo failed to detect T. sp. (buffalo). This strongly suggests that R. appendiculatus may not be a vector for T. sp. (buffalo). Collectively, the data provides further evidence that T. sp. (buffalo). is a distinct species from T. parva.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Weir, Dr William
Authors: Bishop, R.P., Hemmink, J.D., Morrison, W.I., Weir, W., Toye, P.G., Sitt, T., Spooner, P.R., Musoke, A.J., Skilton, R.A., and Odongo, D.O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:International Journal for Parasitology: Parasites and Wildlife
Publisher:Elsevier
ISSN:2213-2244
ISSN (Online):2213-2244
Published Online:29 August 2015
Copyright Holders:Copyright © 2015 Elsevier Ltd.
First Published:First published in International Journal for Parasitology: Parasites and Wildlife 4(3):333-342
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
508691Understanding the basis of strain restricted immunity to Theileria parvaBrian ShielsBiotechnology and Biological Sciences Research Council (BBSRC)BB/H009515/1III - PARASITOLOGY