Extensive modulation of the fecal metagenome in children with Crohn’s Disease during exclusive enteral nutrition

Quince, C. et al. (2015) Extensive modulation of the fecal metagenome in children with Crohn’s Disease during exclusive enteral nutrition. American Journal of Gastroenterology, 110, pp. 1718-1729. (doi:10.1038/ajg.2015.357) (PMID:26526081)

Quince, C. et al. (2015) Extensive modulation of the fecal metagenome in children with Crohn’s Disease during exclusive enteral nutrition. American Journal of Gastroenterology, 110, pp. 1718-1729. (doi:10.1038/ajg.2015.357) (PMID:26526081)

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Abstract

OBJECTIVES: Exploring associations between the gut microbiota and colonic inflammation and assessing sequential changes during exclusive enteral nutrition (EEN) may offer clues into the microbial origins of Crohn’s disease (CD). METHODS: Fecal samples (n=117) were collected from 23 CD and 21 healthy children. From CD children fecal samples were collected before, during EEN, and when patients returned to their habitual diets. Microbiota composition and functional capacity were characterized using sequencing of the 16S rRNA gene and shotgun metagenomics. RESULTS: Microbial diversity was lower in CD than controls before EEN (P=0.006); differences were observed in 36 genera, 141 operational taxonomic units (OTUs), and 44 oligotypes. During EEN, the microbial diversity of CD children further decreased, and the community structure became even more dissimilar than that of controls. Every 10 days on EEN, 0.6 genus diversity equivalents were lost; 34 genera decreased and one increased during EEN. Fecal calprotectin correlated with 35 OTUs, 14 of which accounted for 78% of its variation. OTUs that correlated positively or negatively with calprotectin decreased during EEN. The microbiota of CD patients had a broader functional capacity than healthy controls, but diversity decreased with EEN. Genes involved in membrane transport, sulfur reduction, and nutrient biosynthesis differed between patients and controls. The abundance of genes involved in biotin (P=0.005) and thiamine biosynthesis decreased (P=0.017), whereas those involved in spermidine/putrescine biosynthesis (P=0.031), or the shikimate pathway (P=0.058), increased during EEN. CONCLUSIONS: Disease improvement following treatment with EEN is associated with extensive modulation of the gut microbiome.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Russell, Dr Richard and Barclay, Dr Andrew and Gerasimidis, Dr Konstantinos and Russell, Mrs Julie and Edwards, Professor Christine and Quince, Dr Christopher and Ijaz, Dr Umer Zeeshan and Hansen, Dr Richard and Bertz, Mr Martin
Authors: Quince, C., Ijaz, U. Z., Loman, N., Eren, A. M., Saulnier, D., Russell, J., Haig, S. J., Calus, S. T., Quick, J., Barclay, A., Bertz, M., Blaut, M., Hansen, R., McGrogan, P., Russell, R. K., Edwards, C. A., and Gerasimidis, K.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Infrastructure and Environment
Journal Name:American Journal of Gastroenterology
Publisher:Nature Publishing Group
ISSN:0002-9270
ISSN (Online):1572-0241
Copyright Holders:Copyright © 2015 American College of Gastroenterology
First Published:First published in American Journal of Gastroenterology 110:1718-1729
Publisher Policy:Reproduced under a Creative Commons License

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