The arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis

Ji, H., Wang, Y., Cloix, C., Li, K., Jenkins, G. I. , Wang, S., Shang, Z., Shi, Y., Yang, S. and Li, X. (2015) The arabidopsis RCC1 family protein TCF1 regulates freezing tolerance and cold acclimation through modulating lignin biosynthesis. PLoS Genetics, 11(9), e1005471. (doi:10.1371/journal.pgen.1005471) (PMID:26393916) (PMCID:PMC4579128)

[img]
Preview
Text
111521.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

9MB

Abstract

Cell water permeability and cell wall properties are critical to survival of plant cells during freezing, however the underlying molecular mechanisms remain elusive. Here, we report that a specifically cold-induced nuclear protein, Tolerant to Chilling and Freezing 1 (TCF1), interacts with histones H3 and H4 and associates with chromatin containing a target gene, BLUE-COPPER-BINDING PROTEIN (BCB), encoding a glycosylphosphatidylinositol-anchored protein that regulates lignin biosynthesis. Loss of TCF1 function leads to reduced BCB transcription through affecting H3K4me2 and H3K27me3 levels within the BCB gene, resulting in reduced lignin content and enhanced freezing tolerance. Furthermore, plants with knocked-down BCB expression (amiRNA-BCB) under cold acclimation had reduced lignin accumulation and increased freezing tolerance. The pal1pal2 double mutant (lignin content reduced by 30% compared with WT) also showed the freezing tolerant phenotype, and TCF1 and BCB act upstream of PALs to regulate lignin content. In addition, TCF1 acts independently of the CBF (C-repeat binding factor) pathway. Our findings delineate a novel molecular pathway linking the TCF1-mediated cold-specific transcriptional program to lignin biosynthesis, thus achieving cell wall remodeling with increased freezing tolerance.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cloix, Dr Catherine and Jenkins, Professor Gareth
Authors: Ji, H., Wang, Y., Cloix, C., Li, K., Jenkins, G. I., Wang, S., Shang, Z., Shi, Y., Yang, S., and Li, X.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:PLoS Genetics
Publisher:Public Library of Science
ISSN:1553-7404
ISSN (Online):1553-7404
Copyright Holders:Copyright © 2015 Ji et al.
First Published:First published in PLoS Genetics 11(9):e1005471
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record