Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus

Lecova, L., Růžičková, M., Laing, R. , Vogel, H., Szotáková, B., Prchal, L., Lamka, J., Vokřál, I., Skálová, L. and Matoušková, P. (2015) Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus. Molecular and Biochemical Parasitology, 201(2), pp. 123-127. (doi: 10.1016/j.molbiopara.2015.08.001) (PMID:26255779)

Full text not currently available from Enlighten.

Abstract

The aim of this work was to identify reliable reference genes for expression studies in adult Haemonchus contortus. Eleven candidate genes were identified and the stability of their expression was assessed in adult males and females of two genetically divergent H. contortus isolates: drug-susceptible (ISE) and multi-drug-resistant (WR). Five genes with the most stable expression patterns were further assessed for suitability as reference genes in anthelmintic-treated H. contortus adults versus non-treated controls. We identified important differences in the expression of a number of candidate genes in anthelmintic-treated samples, confirming the need for careful validation of control genes for such experiments. We propose the use of multiple reference genes for expression studies in this species and found gpd, ama and far most suitable for adult H. contortus.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lecova, Dr Lenka and Laing, Dr Roz
Authors: Lecova, L., Růžičková, M., Laing, R., Vogel, H., Szotáková, B., Prchal, L., Lamka, J., Vokřál, I., Skálová, L., and Matoušková, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier
ISSN:0166-6851
ISSN (Online):1872-9428

University Staff: Request a correction | Enlighten Editors: Update this record