Acetylcholinesterase activity as a neurotoxicity marker within the context of experimentally-simulated hyperprolinaemia: an in vitro approach

Kalafatakis, K., Gkanti, V., Mackenzie-Gray Scott, C. A., Zarros, A., Baillie, G. S. and Tsakiris, S. (2015) Acetylcholinesterase activity as a neurotoxicity marker within the context of experimentally-simulated hyperprolinaemia: an in vitro approach. Journal of Natural Science, Biology and Medicine, 6(3), pp. 98-101. (doi:10.4103/0976-9668.166099)

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Abstract

Hyperprolinaemia is characterized by increased tissue accumulation of proline (Pro) and is known to exert serious cognitive and/or neuropsychiatric symptomatology as a direct result of Pro accumulation in the brain. The aim of this study was to explore a putative link between experimentally-simulated hyperprolinaemia and the activity of acetylcholinesterase (AChE); a crucial neurotoxicity marker. In vitro experiments were undertaken on purified eel-derived AChE, as well as on adult mouse brain homogenates, in order to examine the effect of a spectrum of Pro concentrations (3, 30, 500, and 1000 μM) on this marker. Our data showed that although Pro exerted a significant inhibitory effect on pure AChE activity, mouse brain-derived membrane-bound AChE activity was found either unaltered or significantly increased following incubation with Pro. The use of AChE activity as a neurotoxicity marker within the context of experimentally-simulated hyperprolinaemia should be considered with caution and in parallel with a number of other experimental parameters.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Baillie, Professor George and Zarros, Dr Apostolos
Authors: Kalafatakis, K., Gkanti, V., Mackenzie-Gray Scott, C. A., Zarros, A., Baillie, G. S., and Tsakiris, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Journal of Natural Science, Biology and Medicine
Publisher:Wolters Kluwer
ISSN:0976-9668
ISSN (Online):2229-7707
Copyright Holders:Copyright © 2015 Journal of Natural Science, Biology and Medicine
First Published:First published in Journal of Natural Science, Biology and Medicine 6(3):98-101
Publisher Policy:Reproduced under a Creative Commons License

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