Evaluation of platelet activation in canine immune-mediated haemolytic anaemia

Ridyard, A.E. , Shaw, D.J. and Milne, E.M. (2010) Evaluation of platelet activation in canine immune-mediated haemolytic anaemia. Journal of Small Animal Practice, 51(6), pp. 296-304. (doi: 10.1111/j.1748-5827.2010.00935.x) (PMID:20618628)

Abstract

Objectives: To establish whether heightened platelet activation is a common feature of canine immune-mediated haemolytic anaemia, and to evaluate the hypothesis that platelet activation plays a role in the pathogenesis of thromboembolism. Methods: Using whole-blood flow-cytometric analysis, the proportion of activated platelets and platelet-leucocyte aggregates in blood samples from 14 dogs with immune-mediated haemolytic anaemia and 14 healthy dogs was calculated. General linear models with binomial errors were used to compare groups. Results from the immune-mediated haemolytic anaemia-affected dogs were then correlated with established risk factors for thromboembolism in canine immune-mediated haemolytic anaemia, D-dimer concentration and antithrombin activity. Results: There was a strong correlation between platelet activation and severe thrombocytopenia, with heightened platelet activation being observed predominantly in severely thrombocytopenic dogs. Clinical Significance: Dogs with immune-mediated haemolytic anaemia, particularly those with concurrent severe thrombocytopenia, are likely to have heightened platelet activation, which may play a role in the pathogenesis of thromboembolism.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ridyard, Ms Alison
• Authors: Ridyard, A.E., Shaw, D.J., and Milne, E.M.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Small Animal Practice
• Publisher: Wiley-Blackwell Publishing Ltd.
• ISSN: 0022-4510
• ISSN (Online): 1748-5827