Mnatsakanyan, H., Rico, P., Grigoriou, E. , Candelas, A. M., Rodrigo-Navarro, A. , Salmeron-Sanchez, M. and Sabater i Serra, R. (2015) Controlled assembly of fibronectin nanofibrils triggered by random copolymer chemistry. ACS Applied Materials and Interfaces, 7(32), pp. 18125-18135. (doi: 10.1021/acsami.5b05466) (PMID:26225535)
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Abstract
Fibronectin fibrillogenesis is the physiological process by which cells elaborate a fibrous FN matrix. Poly(ethyl acrylate), PEA, has been described to induce a similar process upon simple adsorption of fibronectin (FN) from a protein solution—in the absence of cells—leading to the so-called material-driven fibronectin fibrillogenesis. Poly(methyl acrylate), PMA, is a polymer with very similar chemistry to PEA, on which FN is adsorbed, keeping the globular conformation of the protein in solution. We have used radical polymerization to synthesize copolymers with controlled EA/MA ratio, seeking to modulate the degree of FN fibrillogenesis. The physicochemical properties of the system were studied using dynamic–mechanical analysis, differential scanning calorimetry, and water contact angle. Both the degree of FN fibrillogenesis and the availability of the integrin binding region of FN directly depend on the percentage of EA in the copolymer, whereas the same total amount of FN was adsorbed regardless the EA/MA ratio. Cell morphology adhesion and differentiation of murine C2C12 were shown to depend on the degree of FN fibrillogenesis previously attained on the material surface. Myogenic differentiation was enhanced on the copolymers with higher EA content, i.e. more interconnected FN fibrils.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Salmeron-Sanchez, Professor Manuel and Rodrigo-Navarro, Mr Aleixandre and Grigoriou, Eleni and Rico Tortosa, Dr Patricia |
| Authors: | Mnatsakanyan, H., Rico, P., Grigoriou, E., Candelas, A. M., Rodrigo-Navarro, A., Salmeron-Sanchez, M., and Sabater i Serra, R. |
| College/School: | College of Science and Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | ACS Applied Materials and Interfaces |
| Publisher: | American Chemical Society |
| ISSN: | 1944-8244 |
| ISSN (Online): | 1944-8252 |
| Published Online: | 30 July 2015 |
| Copyright Holders: | Copyright © 2015 American Chemical Society |
| First Published: | First published in ACS Applied Materials and Interfaces 7(32):18125-18135 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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