Dever, G., Stewart, L.-J., Pitt, A. R. and Spickett, C. M. (2003) Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells. FEBS Letters, 540(1-3), pp. 245-250. (doi: 10.1016/S0014-5793(03)00271-0)
Full text not currently available from Enlighten.
Publisher's URL: http://dx.doi.org/10.1016/S0014-5793(03)00271-0
Abstract
Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (HL60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10–100 μM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Pitt, Dr Andrew and Dever, Dr Gary |
| Authors: | Dever, G., Stewart, L.-J., Pitt, A. R., and Spickett, C. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | FEBS Letters |
| Publisher: | Elsevier BV |
| ISSN: | 0014-5793 |
| ISSN (Online): | 1873-3468 |
| Published Online: | 21 March 2003 |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details