Comprehensive LC−MSELipidomic analysis using a shotgun approach and its application to biomarker detection and identification in osteoarthritis patients

Castro-Perez, J. M., Kamphorst, J. , DeGroot, J., Lafeber, F., Goshawk, J., Yu, K., Shockcor, J. P., Vreeken, R. J. and Hankemeier, T. (2010) Comprehensive LC−MSELipidomic analysis using a shotgun approach and its application to biomarker detection and identification in osteoarthritis patients. Journal of Proteome Research, 9(5), pp. 2377-2389. (doi:10.1021/pr901094j) (PMID:20355720)

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Abstract

A “shotgun lipidomic LC−MS approach” was developed by the use of high resolution and exact mass. A total of 284 lipids were identified in human plasma. The method was validated with five nonendogenous lipids, the linearity (r2 > 0.994) and the intraday and interday repeatability (RSD, 4.3−6.2% and 4.8−8%, respectively) were satisfactory. The developed lipid profiling method was applied for the analysis of plasma from humans suffering from osteoarthritis (OA).

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kamphorst, Dr Jurre
Authors: Castro-Perez, J. M., Kamphorst, J., DeGroot, J., Lafeber, F., Goshawk, J., Yu, K., Shockcor, J. P., Vreeken, R. J., and Hankemeier, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Journal of Proteome Research
Publisher:American Chemical Society
ISSN:1535-3893
ISSN (Online):1535-3907

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