A roadmap for interpreting 13C metabolite labeling patterns from cells

Buescher, J. M. et al. (2015) A roadmap for interpreting 13C metabolite labeling patterns from cells. Current Opinion in Biotechnology, 34, pp. 189-201. (doi: 10.1016/j.copbio.2015.02.003) (PMID:25731751) (PMCID:PMC4552607)

Abstract

Measuring intracellular metabolism has increasingly led to important insights in biomedical research. 13C tracer analysis, although less information-rich than quantitative 13C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrient contributions. Here, we review selected key issues in interpreting 13C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiments.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kamphorst, Dr Jurre and Maddocks, Professor Oliver and Gottlieb, Professor Eyal and Vazquez, Alexei and Vousden, Karen
• Authors: Buescher, J. M., Antoniewicz, M. R., Boros, L. G., Burgess, S. C., Brunengraber, H., Clish, C. B., DeBerardinis, R. J., Feron, O., Frezza, C., Ghesquiere, B., Gottlieb, E., Hiller, K., Jones, R. G., Kamphorst, J. J., Kibbey, R. G., Kimmelman, A. C., Locasale, J. W., Lunt, S. Y., Maddocks, O. D.K., Malloy, C., Metallo, C. M., Meuillet, E. J., Munger, J., Nöh, K., Rabinowitz, J. D., Ralser, M., Sauer, U., Stephanopoulos, G., St-Pierre, J., Tennant, D. A., Wittmann, C., Vander Heiden, M. G., Vazquez, A., Vousden, K., Young, J. D., Zamboni, N., and Fendt, S.-M.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Current Opinion in Biotechnology
• Publisher: Elsevier Ltd.
• ISSN: 0958-1669
• ISSN (Online): 1879-0429