Mouse tissue culture models of unstable triplet repeats

Gomes-Pereira, M. and Monckton, D. G. (2004) Mouse tissue culture models of unstable triplet repeats. Methods in Molecular Biology(277), pp. 215-227. (doi: 10.1385/1-59259-804-8:215)

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Once into the expanded disease-associated range, trinucleotide repeat alleles become dramatically unstable in the germline and in somatic cells. The molecular mechanism(s) that underlie this unique form of dynamic mutation are poorly understood. Numerous transgenic mouse models of unstable trinucleotide repeats, which reconstitute the dynamic nature of somatic mosaicism observed in humans, have been generated. Given their easy accessibility, tissues from these mice can be collected to establish homogenous cell culture models of trinucleotide repeat dynamics. This chapter describes how such cultures can be established and maintained. Such in vitro systems may be useful to study relevant biological questions concerning fundamental triplet repeat metabolism. In particular, monitoring of repeat stability in cells growing under controlled conditions could help to clarify the relationship among the accumulation of repeat length variation, cell division rates, and DNA replication.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Monckton, Professor Darren
Authors: Gomes-Pereira, M., and Monckton, D. G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Methods in Molecular Biology
Publisher:Humana Press
ISSN (Online):1940-6029

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