One step closer to understanding the role of bacteria in diabetic foot ulcers: characterising the microbiome of ulcers

Smith, K., Collier, A., Townsend, E., O'Donnell, L., Bal, A., MacKay, W., Ramage, G. and Williams, C. (2016) One step closer to understanding the role of bacteria in diabetic foot ulcers: characterising the microbiome of ulcers. BMC Microbiology, 16(54), (doi:10.1186/s12866-016-0665-z) (PMID:27005417) (PMCID:PMC4804642)

Smith, K., Collier, A., Townsend, E., O'Donnell, L., Bal, A., MacKay, W., Ramage, G. and Williams, C. (2016) One step closer to understanding the role of bacteria in diabetic foot ulcers: characterising the microbiome of ulcers. BMC Microbiology, 16(54), (doi:10.1186/s12866-016-0665-z) (PMID:27005417) (PMCID:PMC4804642)

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Abstract

Background: The aim of this study was to characterise the microbiome of new and recurrent diabetic foot ulcers using 16S amplicon sequencing (16S AS), allowing the identification of a wider range of bacterial species that may be important in the development of chronicity in these debilitating wounds. Twenty patients not receiving antibiotics for the past three months were selected, with swabs taken from each individual for culture and 16S AS. DNA was isolated using a combination of bead beating and kit extraction. Samples were sequenced on the Illumina Hiseq 2500 platform. Results: Conventional laboratory culture showed positive growth from only 55 % of the patients, whereas 16S AS was positive for 75 % of the patients (41 unique genera, representing 82 different operational taxonomic units (OTU’s). S. aureus was isolated in 72 % of culture-positive samples, whereas the most commonly detected bacteria in all ulcers were Peptoniphilusspp., Anaerococcus spp. and Corynebacterium spp., with the addition of Staphylococcus spp. in new ulcers. The majority of OTU’s residing in both new and recurrent ulcers (over 67 %) were identified as facultative or strict anaerobic Gram-positive organisms. Principal component analysis (PCA) showed no difference in clustering between the two groups (new and recurrent ulcers). Conclusions: The abundance of anaerobic bacteria has important implications for treatment as it suggests that the microbiome of each ulcer “starts afresh” and that, although diverse, are not distinctly different from one another with respect to new or recurrent ulcers. Therefore, when considering antibiotic therapy the duration of current ulceration may be a more important consideration than a history of healed ulcer.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:MacKay, Mr William and Ramage, Professor Gordon and Bal, Dr Abhijit and Williams, Dr Craig and Townsend, Miss Eleanor
Authors: Smith, K., Collier, A., Townsend, E., O'Donnell, L., Bal, A., MacKay, W., Ramage, G., and Williams, C.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:BMC Microbiology
Publisher:BioMed Central
ISSN:1471-2180
ISSN (Online):1471-2180
Copyright Holders:Copyright © 2016 Smith et a
First Published:First published in BMC Microbiology 16:54
Publisher Policy:Reproduced under a creative commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
614821The Natural History of Dentures: A Microbiological PerspectiveGordon RamageBiotechnology and Biological Sciences Research Council (BBSRC)BB/K501013/1SM - DENTAL SCHOOL