Exposure of mHIPPOE-14 cells to oligomycin as a tool for in vitro approaches to brain ischaemia-related pro- and epiphenomena: time-dependent cellular response profiling

Zarros, A., Cameron, R.T., Bimpis, A. and Baillie, G.S. (2015) Exposure of mHIPPOE-14 cells to oligomycin as a tool for in vitro approaches to brain ischaemia-related pro- and epiphenomena: time-dependent cellular response profiling. Atherosclerosis, 241(1), e221. (doi:10.1016/j.atherosclerosis.2015.04.1038)

Full text not currently available from Enlighten.

Abstract

Aim: To assess the effect of oligomycin (Figure 1.a) as a tool for the induction of “chemical hypoxia” on an immortalized embryonic murine hippocampal cell-line (mHippoE-14) via the use of xCELLigence technology (that monitors real-time cellular proliferation and cellular events without the incorporation of labels), in the presence and absence of foetal bovine serum (FBS). Methods: xCELLigence monitored mHippoE-14 cells that were seeded at a density of 7.5 x 103 cells/well in 96-well plates and were allowed to grow for 24 h prior to being exposed to oligomycin (1 ug/mL) in the presence and absence of FBS. Parallel phase-contrast microscopy morphological studies were conducted. Results: FBS-deprivation led mHippoE-14 cells into a “pathopoietic phase”. Within this phase, oligomycin exerted a stable and statistically-significant decrease of the cellular index (Figure 1.b; green line) that was associated with both morphological and numerical changes in the exposed neuronal populations; the latter could allow for the use of oligomycin in the in vitro simulation of pro- and epiphenomena related to neuronal ischaemic injury. Conclusions: The highly-reproducible xCELLigence data generated by the exposure of mHippoE-14 to oligomycin have provided us with a number of important observations of technical interest that could allow for a more reliable implementation of the specific cell-line in the in vitro neuropathopoiesis attempts aiming to simulate ischaemic brain injury and related drug-screening assessment.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Baillie, Professor George and Cameron, Mr Ryan
Authors: Zarros, A., Cameron, R.T., Bimpis, A., and Baillie, G.S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Atherosclerosis
Publisher:Elsevier Ltd.
ISSN:0021-9150
ISSN (Online):1879-1484

University Staff: Request a correction | Enlighten Editors: Update this record