Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors

Dent, M. R., López-Duarte, I., Dickson, C. J., Geoghegan, N. D., Cooper, J. M. , Gould, I. R., Krams, R., Bull, J. A., Brooks, N. J. and Kuimova, M. K. (2015) Imaging phase separation in model lipid membranes through the use of BODIPY based molecular rotors. Physical Chemistry Chemical Physics, 17(28), pp. 18393-18402. (doi:10.1039/c5cp01937k) (PMID:26104504)

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Abstract

In order to fully understand the dynamics of processes within biological lipid membranes, it is necessary to possess an intimate knowledge of the physical state and ordering of lipids within the membrane. Here we report the use of three molecular rotors based on meso-substituted boron-dipyrrin (BODIPY) in combination with fluorescence lifetime spectroscopy to investigate the viscosity and phase behaviour of model lipid bilayers. In phase-separated giant unilamellar vesicles, we visualise both liquid-ordered (Lo) and liquid-disordered (Ld) phases using fluorescence lifetime imaging microscopy (FLIM), determining their associated viscosity values, and investigate the effect of composition on the viscosity of these phases. Additionally, we use molecular dynamics simulations to investigate the orientation of the BODIPY probes within the bilayer, as well as using molecular dynamics simulations and fluorescence correlation spectroscopy (FCS) to compare diffusion coefficients with those predicted from the fluorescence lifetimes of the probes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan
Authors: Dent, M. R., López-Duarte, I., Dickson, C. J., Geoghegan, N. D., Cooper, J. M., Gould, I. R., Krams, R., Bull, J. A., Brooks, N. J., and Kuimova, M. K.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Physical Chemistry Chemical Physics
Publisher:Royal Society of Chemistry
ISSN:1463-9076
ISSN (Online):1463-9084

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
553521Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and WellbeingJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/I017887/1ENG - BIOMEDICAL ENGINEERING