The aspergillus fumigatus sitA phosphatase homologue is important for adhesion, cell wall integrity, biofilm formation, and virulence

Bom, V. L. P. et al. (2015) The aspergillus fumigatus sitA phosphatase homologue is important for adhesion, cell wall integrity, biofilm formation, and virulence. Eukaryotic Cell, 14(8), pp. 728-744. (doi:10.1128/EC.00008-15) (PMID:25911225)

Bom, V. L. P. et al. (2015) The aspergillus fumigatus sitA phosphatase homologue is important for adhesion, cell wall integrity, biofilm formation, and virulence. Eukaryotic Cell, 14(8), pp. 728-744. (doi:10.1128/EC.00008-15) (PMID:25911225)

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Abstract

Aspergillus fumigatus is an opportunistic pathogenic fungus able to infect immunocompromised patients, eventually causing disseminated infections that are difficult to control and lead to high mortality rates. It is important to understand how the signaling pathways that regulate these factors involved in virulence are orchestrated. Protein phosphatases are central to numerous signal transduction pathways. Here, we characterize the A. fumigatus protein phosphatase 2A SitA, the Saccharomyces cerevisiae Sit4p homologue. The sitA gene is not an essential gene, and we were able to construct an A. fumigatus null mutant. The ΔsitA strain had decreased MpkA phosphorylation levels, was more sensitive to cell wall-damaging agents, had increased β-(1,3)-glucan and chitin, was impaired in biofilm formation, and had decreased protein kinase C activity. The ΔsitA strain is more sensitive to several metals and ions, such as MnCl2, CaCl2, and LiCl, but it is more resistant to ZnSO4. The ΔsitA strain was avirulent in a murine model of invasive pulmonary aspergillosis and induces an augmented tumor necrosis factor alpha (TNF-α) response in mouse macrophages. These results stress the importance of A. fumigatus SitA as a possible modulator of PkcA/MpkA activity and its involvement in the cell wall integrity pathway.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon and Rajendran, Dr Ranjith
Authors: Bom, V. L. P., de Castro, P. A., Winkelströter, L. K., Marine, M., Hori, J. I., Ramalho, L. N. Z., dos Reis, T. F., Goldman, M. H. S., Brown, N. A., Rajendran, R., Ramage, G., Walker, L. A., Munro, C. A., Rocha, M. C., Malavazi, I., Hagiwara, D., and Goldman, G. H.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Eukaryotic Cell
Publisher:American Society for Microbiology
ISSN:1535-9778
ISSN (Online):1535-9786

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