Aspergillus fumigatus enhances elastase production in pseudomonas aeruginosaco-cultures

Smith, K., Rajendran, R., Kerr, S., Lappin, D. F., Mackay, W. G., Williams, C. and Ramage, G. (2015) Aspergillus fumigatus enhances elastase production in pseudomonas aeruginosaco-cultures. Medical Mycology, 53(7), pp. 645-655. (doi:10.1093/mmy/myv048) (PMID:26162475)

Smith, K., Rajendran, R., Kerr, S., Lappin, D. F., Mackay, W. G., Williams, C. and Ramage, G. (2015) Aspergillus fumigatus enhances elastase production in pseudomonas aeruginosaco-cultures. Medical Mycology, 53(7), pp. 645-655. (doi:10.1093/mmy/myv048) (PMID:26162475)

[img]
Preview
Text
108839.pdf - Accepted Version

154kB

Abstract

In the cystic fibrosis (CF) lung the presence of bacteria and fungi in the airways promotes an inflammatory response causing progressive lung damage, ultimately leading to high rates of morbidity and mortality. We hypothesized that polymicrobial interactions play an important role in promoting airway pathogenesis. We therefore examined the interplay between the most commonly isolated bacterial CF pathogen, Pseudomonas aeruginosa, and the most prevalent filamentous fungi, Aspergillus fumigatus, to test this. Co-culture experiments showed that in the presence of A. fumigatus the production of P. aeruginosa elastase was enhanced. This was confirmed by the presence of zones of clearance on Elastin-Congo Red (ECR) agar, which was identified as elastase by mass spectrometry. When P. aeruginosa were grown in a co-culture model with mature A. fumigatus biofilms, 60% of isolates produced significantly more elastase in the presence of the filamentous fungi than in its absence (P < .05). The expression of lasB also increased when P. aeruginosa isolates PA01 and PA14 were grown in co-culture with A. fumigatus. Supernatants from co-culture experiments were also significantly toxic to a human lung epithelial cell line (19–38% cell cytotoxicity) in comparison to supernatants from P. aeruginosa only cultures (P < .0001). Here we report that P. aeruginosa cytotoxic elastase is enhanced in the presence of the filamentous fungi A. fumigatus, suggesting that this may have a role to play in the damaging pathology associated with the lung tissue in this disease. This indicates that patients who have a co-colonisation with these two organisms may have a poorer prognosis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lappin, Dr David and Ramage, Professor Gordon and Rajendran, Dr Ranjith
Authors: Smith, K., Rajendran, R., Kerr, S., Lappin, D. F., Mackay, W. G., Williams, C., and Ramage, G.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Medical Mycology
Publisher:Oxford University Press
ISSN:1369-3786
ISSN (Online):1460-2709
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Medical Mycology 53(7):645-655
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record