Proton-coupled electron transfer constitutes the photoactivation mechanism of the plant photoreceptor UVR8

Mathes, T. et al. (2015) Proton-coupled electron transfer constitutes the photoactivation mechanism of the plant photoreceptor UVR8. Journal of the American Chemical Society, 137(25), pp. 8113-8120. (doi:10.1021/jacs.5b01177) (PMID:25955727)

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Abstract

UVR8 is a novel UV-B photoreceptor that regulates a range of plant responses and is already used as a versatile optogenetic tool. Instead of an exogenous chromophore, UVR8 uniquely employs tryptophan side chains to accomplish UV-B photoreception. UV-B absorption by homodimeric UVR8 induces monomerization and hence signaling, but the underlying photodynamic mechanisms are not known. Here, by using a combination of time-resolved fluorescence and absorption spectroscopy from femto- to microseconds, we provide the first experimental evidence for the UVR8 molecular signaling mechanism. The results indicate that tryptophan residues at the dimer interface engage in photoinduced proton coupled electron transfer reactions that induce monomerization.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Heilmann, Miss Monika and Smith, Dr Brian and Fu, Dr Yinan and Jenkins, Professor Gareth and Christie, Professor John
Authors: Mathes, T., Heilmann, M., Pandit, A., Zhu, J., Ravensbergen, J., Kloz, M., Fu, Y., Smith, B.O., Christie, J.M., Jenkins, G.I., and Kennis, J.T.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of the American Chemical Society
Publisher:American Chemical Society
ISSN:0002-7863
ISSN (Online):1520-5126

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