Revealing microbial recognition by specific antibodies

Simón-Soro, Á., D’Auria, G., Collado, M. C., Džunková, M., Culshaw, S. and Mira, A. (2015) Revealing microbial recognition by specific antibodies. BMC Microbiology, 15, 132. (doi: 10.1186/s12866-015-0456-y) (PMID:26134992) (PMCID:PMC4489363)

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Background: Recognition of microorganisms by antibodies is a vital component of the human immune response. However, there is currently very limited understanding of immune recognition of 50 % of the human microbiome which is made up of as yet un-culturable bacteria. We have combined the use of flow cytometry and pyrosequencing to describe the microbial composition of human samples, and its interaction with the immune system. Results: We show the power of the technique in human faecal, saliva, oral biofilm and breast milk samples, labeled with fluorescent anti-IgG or anti-IgA antibodies. Using Fluorescence-Activated Cell Sorting (FACS), bacterial cells were separated depending on whether they are coated with IgA or IgG antibodies. Each bacterial population was PCR-amplified and pyrosequenced, characterizing the microorganisms which evade the immune system and those which were recognized by each immunoglobulin. Conclusions: The application of the technique to healthy and diseased individuals may unravel the contribution of the immune response to microbial infections and polymicrobial diseases.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Culshaw, Professor Shauna
Authors: Simón-Soro, Á., D’Auria, G., Collado, M. C., Džunková, M., Culshaw, S., and Mira, A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:BMC Microbiology
Publisher:BioMed Central
ISSN (Online):1471-2180
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in BMC Microbiology 15:132
Publisher Policy:Reproduced under a Creative Commons License

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