A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion

Grefen, C., Karnik, R. , Larson, E., Lefoulon, C., Wang, Y., Waghmare, S., Zhang, B. , Hills, A. and Blatt, M. R. (2015) A vesicle-trafficking protein commandeers Kv channel voltage sensors for voltage-dependent secretion. Nature Plants, 1, 15108. (doi:10.1038/nplants.2015.108)

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Abstract

Growth in plants depends on ion transport for osmotic solute uptake and secretory membrane trafficking to deliver material for wall remodelling and cell expansion. The coordination of these processes lies at the heart of the question, unresolved for more than a century, of how plants regulate cell volume and turgor. Here we report that the SNARE protein SYP121 (SYR1/PEN1), which mediates vesicle fusion at the Arabidopsis plasma membrane, binds the voltage sensor domains (VSDs) of K+ channels to confer a voltage dependence on secretory traffic in parallel with K+ uptake. VSD binding enhances secretion in vivo subject to voltage, and mutations affecting VSD conformation alter binding and secretion in parallel with channel gating, net K+ concentration, osmotic content and growth. These results demonstrate a new and unexpected mechanism for secretory control, in which a subset of plant SNAREs commandeer K+ channel VSDs to coordinate membrane trafficking with K+ uptake for growth.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hills, Mr Adrian and Blatt, Professor Michael and Zhang, Dr Ben and Wang, Dr Yizhou and Grefen, Dr Christopher and Larson, Dr Emily and Karnik, Dr Rucha and Lefoulon, Dr Cecile and Waghmare, Dr Sakharam
Authors: Grefen, C., Karnik, R., Larson, E., Lefoulon, C., Wang, Y., Waghmare, S., Zhang, B., Hills, A., and Blatt, M. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Nature Plants
Publisher:Nature Publishing Group
ISSN:2055-026X
ISSN (Online):2055-0278

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