Refinement of the x-ray structure of the RC LH1 core complex from Rhodopseudomonas palustris by single-molecule spectroscopy

Richter, M.F., Baier, J., Southall, J., Cogdell, R. , Oellerich, S. and Kohler, J. (2007) Refinement of the x-ray structure of the RC LH1 core complex from Rhodopseudomonas palustris by single-molecule spectroscopy. Proceedings of the National Academy of Sciences of the United States of America, 104(51), pp. 20280-20284. (doi:10.1073/pnas.0704599105)

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Abstract

A unique combination of single-molecule spectroscopy with numerical simulations has allowed us to achieve a refined structural model for the bacteriochlorophyll a (BChl a) pigment arrangement in reaction center–light-harvesting 1 core complexes of Rhodopseudomonas palustris. Details in the optical spectra, such as spectral separation and mutual polarizations of spectral bands, are compared with results from numerical simulations for various models of the BChl a arrangement that were all well within the 4.8-Å limit of the accuracy of the available x-ray structure. The experimental data are consistent with a geometry where 15 BChl a dimers, each taken homologous to those from light-harvesting 2 complex from Rhodopseudomonas acidophila, are arranged in an overall elliptical structure featuring a gap on the long side of the ellipse.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard
Authors: Richter, M.F., Baier, J., Southall, J., Cogdell, R., Oellerich, S., and Kohler, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Academy of Sciences
ISSN:0027-8424
ISSN (Online):1091-6490

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