Burch, H. J. , Contera, S. A., de Planque, M. R.R., Grobert, N. and Ryan, J.F. (2008) Doping of carbon nanotubes with nitrogen improves protein coverage whilst retaining correct conformation. Nanotechnology, 19(38), 384001. (doi: 10.1088/0957-4484/19/38/384001) (PMID:21832561)
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Abstract
Relevant parameters for non-covalent protein functionalization of carbon nanotubes are explored. Multiwalled carbon nanotubes are carboxylated and functionalized with metalloproteins. Using atomic force microscopy (AFM) we quantitatively determine that coverage with nitrogen-doped multiwalled carbon nanotubes is superior compared to coverage with un-doped multiwalled carbon nanotubes, due to enhanced carboxylation. Conformational analysis using a combination of AFM, antibody binding assays, circular dichroism and UV–visible spectroscopy demonstrates that the metalloproteins retain their native structure when adsorbed to nitrogen-doped multiwalled carbon nanotubes irrespective of their size, charge or folding motif.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hamnett, Dr Hilary |
| Authors: | Burch, H. J., Contera, S. A., de Planque, M. R.R., Grobert, N., and Ryan, J.F. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | Nanotechnology |
| Publisher: | IOP Publishing Ltd. |
| ISSN: | 0957-4484 |
| ISSN (Online): | 1361-6528 |
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