The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells

Gray, S. R. and Kamolrat, T. (2011) The effect of exercise induced cytokines on insulin stimulated glucose transport in C2C12 cells. Cytokine, 55(2), pp. 221-228. (doi: 10.1016/j.cyto.2011.04.019) (PMID:21600784)

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Skeletal muscle contractile activity increases the production of the myokines interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-15 (IL-15) and also skeletal muscle glucose transport. Previous work has revealed a role for IL-6 in mediating glucose uptake, while research on the physiological roles of IL-8 and IL-15 is not so abundant. In the present study we investigated the effects of different concentrations and combinations of IL-6, IL-8 and IL-15 on insulin stimulated glucose transport in C2C12 cells. Furthermore, we also measured AMPK Thr172 and Akt Ser473 phosphorylation via Western blotting. Exposure to 20 pg/ml of individual cytokines had no affect on glucose transport while 1 ng/ml enhanced (P < 0.05) glucose uptake with IL-6, IL-8 and IL-15, respectively. Moreover, the combinations of IL-8 + IL-6 and IL-15 + IL-6 at both 20 pg/ml and 1 ng/ml stimulated (P < 0.05) glucose transport with IL-8 + IL-15 and IL-8 + IL-6 + IL-15 only increasing (P < 0.05) glucose transport at 1 ng/ml, with no affect observed of these combinations at 20 pg/ml. The changes in glucose transport were all associated with an increase (P < 0.05) in AMPK Thr172 phosphorylation with no changes in Akt Ser473 phosphorylation. These findings demonstrated that the exercise induced myokines IL-6, IL-8 and IL-15 enhance glucose transport at 1 ng/ml, with changes only seen at 20 pg/ml with certain myokine combinations. Furthermore these changes in insulin stimulated glucose transport were associated with increased AMPK phosphorylation.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Gray, Dr Stuart
Authors: Gray, S. R., and Kamolrat, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Cytokine
ISSN (Online):1096-0023

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