Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle

Kamolrat, T., Gray, S. R. and Thivierge, M. C. (2013) Fish oil positively regulates anabolic signalling alongside an increase in whole-body gluconeogenesis in ageing skeletal muscle. European Journal of Nutrition, 52(2), pp. 647-657. (doi:10.1007/s00394-012-0368-7) (PMID:22581256)

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Abstract

Purpose: Fish oil, containing mainly long-chain n-3 polyunsaturated fatty acids (LCn-3PUFA), has been found to acutely stimulate protein synthesis and insulin-mediated glucose metabolism. However, the underlying mechanism and more prolonged effect of fish oil during ageing remain to be determined.<p></p> Methods: Fish oil (EPAX6000; 49.6 % eicosapentaenoic acid, 50.4 % docosahexaenoic acid) or control oil (60 % olive, 40 % soy) supplementation was delivered, via chocolate-derived sweets, to rats for 8 weeks. Throughout the study, food intake and body weight were recorded and body composition was investigated using EchoMRI. During the last 40 min of a 6 h infusion, with labelled dextrose ([U-13C]glucose) and amino acids ([1-13C]phenylalanine), blood samples were collected to assess glucose and phenylalanine kinetics. Soleus and longissimus dorsi muscles were extracted for protein and mRNA analyses.<p></p> Results: Fish oil had no effect on food intake or body composition. An increased whole-body glucose turnover, mainly accounted for via an increase in endogenous glucose production, was observed with fish oil feeding. No effects on whole-body phenylalanine turnover were observed. In longissimus dorsi, fish oil augmented the phosphorylation of phosphoinositide 3-kinase (PI3K)[Tyr458] (P = 0.04) and 70 kDa ribosomal protein S6 kinase (p70s6k)[Thr389] (P = 0.04). There were no differences in protein kinase B (Akt)[Ser473], mammalian target of rapamycin (mTOR)[Ser2448], protein phosphatase 2A (PP2A) 56 kDa regulatory B subunit γ (PP2A-B56-γ), forkhead box containing proteins O-subclass 3a (FOX03a)[Ser253] or inflammatory markers (Interleukin-6, Interleukin-1 β, tumour necrosis factor-α, and cyclooxygenase-2).<p></p> Conclusions: Our data suggest that the fish oil may stimulate endogenous glucose production and increase anabolic signalling in ageing rats.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gray, Dr Stuart
Authors: Kamolrat, T., Gray, S. R., and Thivierge, M. C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:European Journal of Nutrition
Publisher:Springer-Verlag
ISSN:1436-6207
ISSN (Online):1436-6215

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