The organisation of spinoparabrachial neurons in the mouse

Cameron, D., Gutierrez-Mecinas, M. , Gomez-Lima, M., Watanabe, M., Polgár, E. and Todd, A. J. (2015) The organisation of spinoparabrachial neurons in the mouse. Pain, 156(10), pp. 2061-2071. (doi: 10.1097/j.pain.0000000000000270) (PMID:26101837) (PMCID:PMC4770364)

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Abstract

The anterolateral tract (ALT), which originates from neurons in lamina I and the deep dorsal horn, represents a major ascending output through which nociceptive information is transmitted to brain areas involved in pain perception. Although there is detailed quantitative information concerning the ALT in the rat, much less is known about this system in the mouse, which is increasingly being used for studies of spinal pain mechanisms because of the availability of genetically modified lines. The aim of this study was therefore to determine the extent to which information about the ALT in the rat can be extrapolated to the mouse. Our results suggest that as in the rat, most lamina I ALT projection neurons in the lumbar enlargement can be retrogradely labelled from the lateral parabrachial area, that the great majority of these cells (~90%) express the neurokinin 1 receptor (NK1r), and that these are larger than other NK1r-expressing neurons in this lamina. This means that many lamina I spinoparabrachial cells can be identified in NK1r-immunostained sections from animals that have not received retrograde tracer injections. However, we also observed certain species differences, in particular we found that many spinoparabrachial cells in lamina III-IV lack the NK1r, meaning that they cannot be identified based solely on expression of this receptor. We also provide evidence that the vast majority of spinoparabrachial cells are glutamatergic, and that some express substance P. These findings will be important for studies designed to unravel the complex neuronal circuitry that underlies spinal pain processing.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Beresford-Polgar, Dr Erika and Todd, Professor Andrew and Gutierrez-Mecinas, Dr Maria
Authors: Cameron, D., Gutierrez-Mecinas, M., Gomez-Lima, M., Watanabe, M., Polgár, E., and Todd, A. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Pain
Publisher:Lippincott Williams & Wilkins
ISSN:0304-3959
ISSN (Online):1872-6623
Copyright Holders:Copyright © 2015 International Association for the Study of Pain
First Published:First published in Pain 2015
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
627371Spinal inhibitory interneurons that suppress itchAndrew ToddMedical Research Council (MRC)MR/L003430/1INP - CENTRE FOR NEUROSCIENCE
644161Defining pain circuitry in health and diseaseAndrew ToddWellcome Trust (WELLCOME)102645/Z/13/ZINP - CENTRE FOR NEUROSCIENCE
644162Defining pain circuitry in health and diseaseAndrew ToddWellcome Trust (WELLCOME)102645/Z/13/ZINP - CENTRE FOR NEUROSCIENCE