Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR

Schneider, P., Reece, S. E., van Schaijk, B. C.L., Bousema, T., Lanke, K. H.W., Meaden, C. S.J., Gadalla, A., Ranford-Cartwright, L. C. and Babiker, H. A. (2015) Quantification of female and male Plasmodium falciparum gametocytes by reverse transcriptase quantitative PCR. Molecular and Biochemical Parasitology, 199(1-2), pp. 29-33. (doi:10.1016/j.molbiopara.2015.03.006) (PMID:25827756)

[img]
Preview
Text
107662.pdf - Published Version
Available under License Creative Commons Attribution.

413kB

Abstract

The transmission of malaria parasites depends on the presence of sexual stages (gametocytes) in the blood, making the ratio and densities of female and male gametocytes important determinants of parasite fitness. This manuscript describes the development of reverse transcriptase quantitative PCR (RT-qPCR) assays to separately quantify mature female and male gametocytes of the human malaria parasite Plasmodium falciparum, and reveals that Pfs25 mRNA is expressed only in female gametocytes. The female (Pfs25) and male (Pfs230p) gametocyte specific RT-qPCR assays have lower detection limits of 0.3 female and 1.8 male gametocytes per microlitre of blood, respectively, making them more sensitive than microscopy. Accurate quantification of the ratio and densities of female and male gametocytes will increase understanding of P. falciparum transmission and improve the evaluation of transmission blocking interventions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ranford-Cartwright, Dr Lisa and Meaden, Ms Cora
Authors: Schneider, P., Reece, S. E., van Schaijk, B. C.L., Bousema, T., Lanke, K. H.W., Meaden, C. S.J., Gadalla, A., Ranford-Cartwright, L. C., and Babiker, H. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier B.V.
ISSN:0166-6851
ISSN (Online):1872-9428
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Molecular and Biochemical Parasitology 199(1-2):29-33
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
534421Genetic analysis of growth rates in Plasmodium falciparumLisa Ranford-CartwrightWellcome Trust (WELLCOME)091791/Z/10/ZIII - PARASITOLOGY
395071Identification of Plasmodium falciparum QTL determining differential infectivity to Anopheles mosquitoesLisa Ranford-CartwrightWellcome Trust (WELLCOME)078749/Z/05/ZIII - PARASITOLOGY