Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis

Ward, R. J., Pediani, J. D. , Godin, A. G. and Milligan, G. (2015) Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis. Journal of Biological Chemistry, 290(20), pp. 12844-12857. (doi:10.1074/jbc.M115.644724) (PMID:25825490) (PMCID:PMC4432300)

[img]
Preview
Text
107197.pdf - Published Version
Available under License Creative Commons Attribution.

2MB

Abstract

Background: The quaternary organization of G protein-coupled receptors remains a subject of considerable debate. Results: The serotonin 5-HT2C receptor is a mixture of monomers, dimers, and higher-order oligomers. These become predominantly monomers upon antagonist treatment. Conclusion: SpIDA indicates that the 5-HT2C receptor exists as mixtures of forms that are regulated in an antagonist-dependent manner. Significance: SpIDA delivers new insights into receptor quaternary structure.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Milligan, Professor Graeme and Pediani, Dr John and Ward, Dr Richard
Authors: Ward, R. J., Pediani, J. D., Godin, A. G., and Milligan, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Journal of Biological Chemistry
Journal Abbr.:J Biol Chem.
Publisher:American Society for Biochemistry and Molecular Biology, Inc.
ISSN:0021-9258
ISSN (Online):1083-351X
First Published:First published in Journal of Biological Chemistry 290(20):12844-12857
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656901The organisational structure of class A GPCRs: Implications for pharmacology, function and therapeutic regulationGraeme MilliganMedical Research Council (MRC)MR/L023806/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
510631The organisational structure of class A GPCRs: implications for function and drug designGraeme MilliganMedical Research Council (MRC)G0900050RI NEUROSCIENCE & PSYCHOLOGY