Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples

Tveen-Jensen, K., Gesellchen, F., Wilson, R. , Spickett, C. M., Cooper, J. M. and Pitt, A. R. (2015) Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples. Scientific Reports, 5, 9736. (doi:10.1038/srep09736) (PMID:25978651) (PMCID:PMC4432867)

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Abstract

Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Dr Robert and Gesellchen, Dr Frank and Cooper, Professor Jonathan
Authors: Tveen-Jensen, K., Gesellchen, F., Wilson, R., Spickett, C. M., Cooper, J. M., and Pitt, A. R.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2015 The Authors
First Published:First published Scientific Reports 5:9736
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
553521Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and WellbeingJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/I017887/1ENG - BIOMEDICAL ENGINEERING
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING