Tveen-Jensen, K., Gesellchen, F., Wilson, R. , Spickett, C. M., Cooper, J. M. and Pitt, A. R. (2015) Interfacing low-energy SAW nebulization with liquid chromatography-mass spectrometry for the analysis of biological samples. Scientific Reports, 5, 9736. (doi: 10.1038/srep09736) (PMID:25978651) (PMCID:PMC4432867)
|
Text
107178.pdf - Updated Version Available under License Creative Commons Attribution. 1MB |
Abstract
Soft ionization methods for the introduction of labile biomolecules into a mass spectrometer are of fundamental importance to biomolecular analysis. Previously, electrospray ionization (ESI) and matrix assisted laser desorption-ionization (MALDI) have been the main ionization methods used. Surface acoustic wave nebulization (SAWN) is a new technique that has been demonstrated to deposit less energy into ions upon ion formation and transfer for detection than other methods for sample introduction into a mass spectrometer (MS). Here we report the optimization and use of SAWN as a nebulization technique for the introduction of samples from a low flow of liquid, and the interfacing of SAWN with liquid chromatographic separation (LC) for the analysis of a protein digest. This demonstrates that SAWN can be a viable, low-energy alternative to ESI for the LC-MS analysis of proteomic samples.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gesellchen, Dr Frank and Wilson, Dr Robert and Cooper, Professor Jonathan |
Authors: | Tveen-Jensen, K., Gesellchen, F., Wilson, R., Spickett, C. M., Cooper, J. M., and Pitt, A. R. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Scientific Reports |
Publisher: | Nature Publishing Group |
ISSN: | 2045-2322 |
ISSN (Online): | 2045-2322 |
Copyright Holders: | Copyright © 2015 The Authors |
First Published: | First published Scientific Reports 5:9736 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record