Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data

Orton, R. J. , Wright, C. F., Morelli, M. J., King, D. J., Paton, D., King, D. and Haydon, D. T. (2015) Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data. BMC Genomics, 16, 229. (doi:10.1186/s12864-015-1456-x) (PMID:25886445) (PMCID:PMC4425905)

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Abstract

There is a high prevalence of coronary artery disease (CAD) in patients with left bundle branch block (LBBB); however there are many other causes for this electrocardiographic abnormality. Non-invasive assessment of these patients remains difficult, and all commonly used modalities exhibit several drawbacks. This often leads to these patients undergoing invasive coronary angiography which may not have been necessary. In this review, we examine the uses and limitations of commonly performed non-invasive tests for diagnosis of CAD in patients with LBBB.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Haydon, Professor Daniel and King, Dr Donald and Orton, Dr Richard and Paton, Professor David and Morelli, Dr Marco
Authors: Orton, R. J., Wright, C. F., Morelli, M. J., King, D. J., Paton, D., King, D., and Haydon, D. T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:BMC Genomics
Publisher:Biomed Central
ISSN:1471-2164
ISSN (Online):1471-2164
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in BMC Genomics 16:229
Publisher Policy:Reproduced under a Creative Commons License

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