Activity of 3β-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin

Şimsek, Ö. and Mihm Carmichael, M. (2014) Activity of 3β-hydroxysteroid dehydrogenase associated with progesterone production in bovine granulosa cells cultured under different concentrations of serum, insulin-like growth factor I, and gonadotropin. Turkish Journal of Veterinary and Animal Sciences, 38(4), pp. 358-362. (doi: 10.3906/vet-1311-57)

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Abstract

Three-β-hydroxysteroid dehydrogenase (3β-HSD) is the enzyme responsible for progesterone production. This study aimed to determine whether 3β-HSD activity can be shown to reflect progesterone production by granulosa cells cultured under different serum conditions and follicle stimulating hormone (FSH), luteinising hormone (LH), and insulin-like growth factor I (IGF-I) concentrations. Large bovine follicles were dissected from abattoir ovaries to recover granulosa cells. Cells were washed, stained for viability, and plated for 48 h in basic medium with or without 5% foetal calf serum (FCS). Subsequently, cells were exposed to FSH (1 ng/mL), LH (10 or 100 ng/mL), or FSH (1 ng/mL) + IGF-I (1 or 10 ng/mL) in a serum-free medium for another 96 h to predict degree of luteinisation. Before and after incubation, granulosa cells were stained for 3β-HSD activity. The high dose of IGF-I (10 ng/mL) increased (P < 0.05) progesterone secretion over 2.5-fold compared with FSH alone or the low dose of IGF-I (1 ng/mL) in cells preincubated with FCS. This was clearly reflected by darker 3β-HSD staining than in cells exposed to FSH and low dose IGF-I.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mihm Carmichael, Dr Monika
Authors: Şimsek, Ö., and Mihm Carmichael, M.
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Turkish Journal of Veterinary and Animal Sciences
Publisher:Scientific and Technical Research Council of Turkey
ISSN:1300-0128
ISSN (Online):1303-6181
Copyright Holders:Copyright © 2015 TÜBİTAK
First Published:First published in Turkish Journal of Veterinary and Animal Sciences 38(4):358-362
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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