PEG molecular net-cloth grafted on polymeric substrates and its bio-merits

Zhao, C., Lin, Z., Yin, H. , Ma, Y., Xu, F. and Yang, W. (2014) PEG molecular net-cloth grafted on polymeric substrates and its bio-merits. Scientific Reports, 4, 4982. (doi: 10.1038/srep04982) (PMID:24845078) (PMCID:PMC4028697)

Abstract

Polymer brushes and hydrogels are sensitive to the environment, which can cause uncontrolled variations on their performance. Herein, for the first time, we report a non-swelling “PEG molecular net-cloth” on a solid surface, fabricated using a novel “visible light induced surface controlled graft cross-linking polymerization” (VSCGCP) technique. Via this method, we show that 1) the 3D-network structure of the net-cloth can be precisely modulated and its thickness controlled; 2) the PEG net-cloth has excellent resistance to non-specific protein adsorption and cell adhesion; 3) the mild polymerization conditions (i.e. visible light and room temperature) provided an ideal tool for in situ encapsulation of delicate biomolecules such as enzymes; 4) the successive grafting of reactive three-dimensional patterns on the PEG net-cloth enables the creation of protein microarrays with high signal to noise ratio. Importantly, this strategy is applicable to any C-H containing surface, and can be easily tailored for a broad range of applications.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Yin, Professor Huabing
• Authors: Zhao, C., Lin, Z., Yin, H., Ma, Y., Xu, F., and Yang, W.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Scientific Reports
• Publisher: Nature Publishing Group
• ISSN: 2045-2322
• ISSN (Online): 2045-2322
• Copyright Holders: Copyright © 2014 The Authors
• First Published: First published in Scientific Reports 4:4982
• Publisher Policy: Reproduced under a Creative Commons License