Low-resolution reconstruction of a synthetic DNA Holliday junction

Nöllmann, M., Stark, W. M. and Byron, O. (2004) Low-resolution reconstruction of a synthetic DNA Holliday junction. Biophysical Journal, 86(5), pp. 3060-3069. (doi: 10.1016/S0006-3495(04)74355-6) (PMID:15111420) (PMCID:PMC1304172)

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We have studied the low-resolution solution conformation of a Holliday (or four-way) DNA junction by using small-angle x-ray scattering, sedimentation velocity, and computational modeling techniques. The scattering data were analyzed in two independent ways: firstly, by rigid-body modeling of the scattering data using previously suggested models for the Holliday junction (HJ), and secondly, by ab initio reconstruction methods. The models found by both methods agree with experimentally determined sedimentation coefficients and are compatible with the results of previous studies using different techniques, but provide a more direct and accurate determination of the solution conformation of the HJ. Our results show that addition of Mg2+ alters the conformation of the HJ from an extended to a stacked arrangement.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Byron, Professor Olwyn and Stark, Professor Marshall and Nollmann, Marcelo
Authors: Nöllmann, M., Stark, W. M., and Byron, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Biophysical Journal
Publisher:Elsevier (Cell Press)
ISSN (Online):1542-0086

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