Antigen-specific chemotaxis of B cells

Komai-Koma, M., Donachie, A.M. and Wilkinson, P.C. (1997) Antigen-specific chemotaxis of B cells. Immunology, 91(4), pp. 579-585. (doi:10.1046/j.1365-2567.1997.00300.x) (PMID:9378498) (PMCID:PMC1363879)

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The chemoattractant effect of soluble protein antigens for B cells from immunized mice was examined. Mice were immunized either via the footpad with ovalbumin (OVA) in complete Freund’s adjuvant (CFA) or with CFA alone; or intraperitoneally with OVA incorporated in immune-stimulating complexes (OVA–ISCOM) or with saline. After 8–14 days B cells were purified from the spleens or the draining popliteal nodes and tested in vitro for locomotor responses to antigen using polarization (shape-change) and filter assays. Cells obtained by both routes of immunization, but not cells from control mice, gave locomotor responses to OVA. Responses were seen in B cells directly after preparation (5–8% of cells responding) but were enhanced if the cells were cultured overnight in the presence of interleukin-4 (IL-4) before testing (10–12% of cells responding). Checkerboard filter assays suggested that the response to OVA was chemotactic. The response was antigen specific since cells from OVA-immunized mice did not respond to bovine serum albumin (BSA), and cells from BSA-immunized mice responded to BSA but not to OVA. The response to OVA was inhibited by preincubation of OVA with anti-OVA but not with anti-BSA. Many of the cells that polarized in response to antigen were larger than any B cells found in control populations suggesting that the responsive cells are those that had been stimulated to enter cell cycle following immunization.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Komai-Koma, Dr Mousa and Donachie, Ms Anne Marie
Authors: Komai-Koma, M., Donachie, A.M., and Wilkinson, P.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Immunology
ISSN (Online):1365-2567

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