LOV-based reporters for fluorescence imaging

Buckley, A. M., Petersen, J., Roe, A. J. , Douce, G. R. and Christie, J. M. (2015) LOV-based reporters for fluorescence imaging. Current Opinion in Chemical Biology, 27, pp. 39-45. (doi:10.1016/j.cbpa.2015.05.011) (PMID:26087123)

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Abstract

Chromophore-binding domains from plant and bacterial photoreceptor proteins have recently gathered increasing attention as new sources of genetically encoded fluorescent proteins (FPs). In particular, FPs based on the flavin-binding LOV (light, oxygen, or voltage sensing) domain offer advantages over green fluorescent protein (GFP) owing to their smaller size, pH and thermal stability, utility under anaerobic conditions and their ability to generate reactive oxygen species. This review focuses on the potential applications of this emerging class of fluorescent reporters, discusses the advantages and limitations of LOV-based FPs, whilst offering insights regarding the further development of this technology for bioimaging and photodynamic therapy.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Roe, Professor Andrew and Petersen, Dr Jan and Douce, Dr Gillian and Buckley, Dr Anthony and Christie, Professor John
Authors: Buckley, A. M., Petersen, J., Roe, A. J., Douce, G. R., and Christie, J. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Current Opinion in Chemical Biology
Publisher:Elsevier Ltd., Current Opinion Journals
ISSN:1367-5931
ISSN (Online):1879-0402
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Current Opinion in Chemical Biology
Publisher Policy:Reproduced under a Creative Commons License 27:39-45

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
581491Regulation of Auxin Fluxes Required For Phototropic GrowthJohn ChristieBiotechnology and Biological Sciences Research Council (BBSRC)BB/J016047/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
608111Modulation of Phytochrome B Signalling by PhosphorylationJohn ChristieBiotechnology and Biological Sciences Research Council (BBSRC)BB/K008129/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
659801Photoreceptor Engineering to Modulate Plant GrowthJohn ChristieBiotechnology and Biological Sciences Research Council (BBSRC)BB/M002128/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
492111Genetic and phenotypic characterisation of emerging virulent Clostridium difficileGillian DouceWellcome Trust (WELLCOME)086418/B/08/ZIII - BACTERIOLOGY