Differential expression of microRNA-206 and its target genes in pre-eclampsia

Akehurst, C. et al. (2015) Differential expression of microRNA-206 and its target genes in pre-eclampsia. Journal of Hypertension, 33(10), pp. 2068-2074. (doi:10.1097/HJH.0000000000000656) (PMID:26213997) (PMCID:PMC4570688)

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Abstract

Objectives: Pre-eclampsia is a multi-system disease that significantly contributes to maternal and fetal morbidity and mortality. In this study, we used a non-biased microarray approach to identify novel circulating miRNAs in maternal plasma that may be associated with pre-eclampsia. Methods: Plasma samples were obtained at 16 and 28 weeks of gestation from 18 women who later developed pre-eclampsia (cases) and 18 matched women with normotensive pregnancies (controls). We studied miRNA expression profiles in plasma and subsequently confirmed miRNA and target gene expression in placenta samples. Placental samples were obtained from an independent cohort of 19 women with pre-eclampsia matched with 19 women with normotensive pregnancies. Results: From the microarray, we identified 1 miRNA that was significantly differentially expressed between cases and controls at 16 weeks of gestation and 6 miRNAs that were significantly differentially expressed at 28 weeks. Following qPCR validation only one, miR-206, was found to be significantly increased in 28 week samples in women who later developed pre-eclampsia (1.4 fold change ± 0.2). The trend for increase in miR-206 expression was mirrored within placental tissue from women with pre-eclampsia. In parallel, IGF-1, a target gene of miR-206, was also found to be down-regulated (0.41 ± 0.04) in placental tissue from women with pre-eclampsia. miR-206 expression was also detectable in myometrium tissue and trophoblast cell lines. Conclusions: Our pilot study has identified miRNA-206 as a novel factor up-regulated in pre-eclampsia within the maternal circulation and in placental tissue.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Freeman, Dr Dilys and McBride, Dr Martin and Delles, Professor Christian and Beattie, Mrs Wendy and Sharafetdinova, Miss Liliia and Brown, Dr Catriona and Work, Dr Lorraine and Robinson, Mr Scott and Carty, Dr David and McClure, Dr John and Akehurst, Dr Christine
Authors: Akehurst, C., Small, H., Sharafetdinova, L., Forrest, R., Beattie, W., Brown, C. E., Robinson, S. W., McClure, J. D., Work, L. M., Carty, D. M., McBride, M. W., Freeman, D. J., and Delles, C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of Hypertension
Publisher:Lippincott Williams & Wilkins
ISSN:0263-6352
ISSN (Online):1473-5598
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Journal of Hypertension 33(10):2068-2074
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
544461MRC Doctoral Training Grant 2010-2014Mary GoodmanMedical Research Council (MRC)G1000389-1/1VICE PRINCIPAL RESEARCH & ENTERPRISE