Single molecule analysis of Trypanosoma brucei DNA replication dynamics

Calderano, S.G., Drosopoulos, W.C., Quaresma, M. M., Marques, C.A. , Kosiyatrakul, S., McCulloch, R. , Schildkraut, C.L. and Elias, M.C. (2015) Single molecule analysis of Trypanosoma brucei DNA replication dynamics. Nucleic Acids Research, 43(5), pp. 2655-2665. (doi: 10.1093/nar/gku1389) (PMID:25690894) (PMCID:PMC4357695)

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Abstract

Eukaryotic genome duplication relies on origins of replication, distributed over multiple chromosomes, to initiate DNA replication. A recent genome-wide analysis of Trypanosoma brucei, the etiological agent of sleeping sickness, localized its replication origins to the boundaries of multigenic transcription units. To better understand genomic replication in this organism, we examined replication by single molecule analysis of replicated DNA. We determined the average speed of replication forks of procyclic and bloodstream form cells and we found that T. brucei DNA replication rate is similar to rates seen in other eukaryotes. We also analyzed the replication dynamics of a central region of chromosome 1 in procyclic forms. We present evidence for replication terminating within the central part of the chromosome and thus emanating from both sides, suggesting a previously unmapped origin toward the 5′ extremity of chromosome 1. Also, termination is not at a fixed location in chromosome 1, but is rather variable. Importantly, we found a replication origin located near an ORC1/CDC6 binding site that is detected after replicative stress induced by hydroxyurea treatment, suggesting it may be a dormant origin activated in response to replicative stress. Collectively, our findings support the existence of more replication origins in T. brucei than previously appreciated.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:De Almeida Marques, Dr Catarina and McCulloch, Professor Richard
Authors: Calderano, S.G., Drosopoulos, W.C., Quaresma, M. M., Marques, C.A., Kosiyatrakul, S., McCulloch, R., Schildkraut, C.L., and Elias, M.C.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN:0305-1048
ISSN (Online):1362-4962
Published Online:17 February 2015
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Nucleic Acids Research 43(5):2655-2655
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
463981Characterisation of Orc1/Cdc6 and DNA replication initiation in Trypanosoma brucei.Richard MccullochWellcome Trust (WELLCOME)083485/Z/07/ZIII - PARASITOLOGY
371796The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/Z/08/ZIII - PARASITOLOGY
371798The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOME)085349/B/08/ZIII - PARASITOLOGY