Periodontitis in the absence of B cells and specific anti-bacterial antibody

Oliver-Bell, J., Butcher, J.P., Malcolm, J. , Macleod, M.K.L. , Adrados Planell, A., Campbell, L., Nibbs, R.J.B. , Garside, P. , McInnes, I.B. and Culshaw, S. (2015) Periodontitis in the absence of B cells and specific anti-bacterial antibody. Oral Microbiology and Immunology, 30(2), pp. 160-169. (doi:10.1111/omi.12082) (PMID:25219939)

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Abstract

Periodontitis (PD) results from complex interactions between a dysbiotic oral microbiota and a dysregulated host immune response. The inflammatory infiltrate in the gingiva of PD patients includes an abundance of B cells, implicating these cells in the immunopathology. We sought to investigate the role of B cells in PD using a murine model. Wild-type or B-cell-deficient (μMT) mice were orally infected with Porphyromonas gingivalis. One or six weeks following infection, lymphocyte populations in the gingiva and cervical draining lymph nodes (dLN) were analysed by flow cytometry; serum anti-P. gingivalis IgG antibody titers were measured by enzyme-linked immunosorbent assay, and alveolar bone loss was determined. In wild-type mice, the percentage of gingival B cells expressing receptor activator of nuclear factor-κB ligand (RANKL) was significantly increased 1 week post-infection (5.36% control versus 11% PD, P < 0.01). The percentage of Fas+ GL7+ germinal centre B cells in the dLN was significantly increased at both 1 week (2.03% control versus 6.90% PD, P < 0.01) and 6 weeks (4.45% control versus 8.77% PD, P < 0.05) post-infection. B-cell-deficient mice were protected from P. gingivalis-induced alveolar bone loss, with a lack of B-cell proliferation and lack of CD4+ CD44+ CD62L− T-cell generation in the dLN, and absence of serum anti-P. gingivalis antibodies. Our data imply a pathological role for B cells in PD, and that selective targeting of this immune axis may have a role in treating severe periodontal disease.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInnes, Professor Iain and Oliver-Bell, Dr Jessica and Nibbs, Professor Robert and Garside, Professor Paul and Malcolm, Dr Jennifer and Butcher, Mr John and Culshaw, Dr Shauna and Adrados Planell, Miss Ana and Macleod, Dr Megan
Authors: Oliver-Bell, J., Butcher, J.P., Malcolm, J., Macleod, M.K.L., Adrados Planell, A., Campbell, L., Nibbs, R.J.B., Garside, P., McInnes, I.B., and Culshaw, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Oral Microbiology and Immunology
Publisher:John Wiley & Sons Ltd.
ISSN:2041-1006
ISSN (Online):2041-1014

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