GHAFOURI, K., Cooney, J., Bedford, D. K., Wilson, J., Caslake, M. J. and Gill, J. M.R. (2015) Moderate exercise increases affinity of large very low density lipoproteins for hydrolysis by lipoprotein lipase. Journal of Clinical Endocrinology and Metabolism, 100(6), pp. 2205-2213. (doi: 10.1210/jc.2015-1196) (PMID:25867814)
|
Text
105439.pdf - Accepted Version 265kB |
Abstract
Context: Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations but the mechanisms responsible are unclear. Objective: To determine the effects of exercise on affinity of chylomicrons, large very low density lipoproteins (VLDL1) and smaller VLDL (VLDL2) for lipoprotein lipase (LPL) mediated TG hydrolysis. Design: Within-participant cross-over study. Setting: A University metabolic investigation unit. Participants: Ten overweight/obese men. Interventions: Participants undertook two oral fat tolerance tests, separated by 7–14 days, in which they had blood taken fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal oxygen uptake (EX); no exercise was performed before the control test (CON). Main outcome measures: Circulating TG-rich lipoprotein concentrations; affinity of chylomicrons, VLDL1, VLDL2 for LPL-mediated TG hydrolysis. Results: Exercise significantly reduced fasting VLDL1-TG concentration (CON: 0.49(0.33–0.72) mmol.l−1, EX: 0.36(0.22–0.59) mmol.l−1, [geometric means (95% confidence interval)]; p=0.04). Time-averaged postprandial chylomicron-TG (CON: 0.55±0.10 mmol.l−1, EX: 0.39±0.08 mmol.l−1, [mean±SEM], p=0.03) and VLDL1-TG (CON: 0.85±0.13 mmol.l−1, EX: 0.66±0.10 mmol.l−1, p=0.01) concentrations were both lower in EX than CON. Affinity of VLDL1 for LPL-mediated TG hydrolysis increased by 2.2(1.3–3.7) fold (geometric mean (95% confidence interval)) (p=0.02) in the fasted state and 2.6(1.8–2.6) fold (p=0.001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials. Conclusions: Exercise increases affinity of VLDL1 for LPL-mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to exercise's TG-lowering effect.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gill, Professor Jason and Bedford, Mrs Dorothy and Cooney, Mrs Josephine and Caslake, Professor Muriel and GHAFOURI, Khloud and Wilson, Mr John |
Authors: | GHAFOURI, K., Cooney, J., Bedford, D. K., Wilson, J., Caslake, M. J., and Gill, J. M.R. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
Journal Name: | Journal of Clinical Endocrinology and Metabolism |
Publisher: | The Endocrine Society |
ISSN: | 0021-972X |
ISSN (Online): | 1945-7197 |
Copyright Holders: | Copyright © 2015 The Endocrine Society |
First Published: | First published in Journal of Clinical Endocrinology and Metabolism |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
University Staff: Request a correction | Enlighten Editors: Update this record