Moderate exercise increases affinity of large very low density lipoproteins for hydrolysis by lipoprotein lipase

GHAFOURI, K., Cooney, J., Bedford, D. K., Wilson, J., Caslake, M. J. and Gill, J. M.R. (2015) Moderate exercise increases affinity of large very low density lipoproteins for hydrolysis by lipoprotein lipase. Journal of Clinical Endocrinology and Metabolism, 100(6), pp. 2205-2213. (doi:10.1210/jc.2015-1196) (PMID:25867814)

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Abstract

Context: Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations but the mechanisms responsible are unclear. Objective: To determine the effects of exercise on affinity of chylomicrons, large very low density lipoproteins (VLDL1) and smaller VLDL (VLDL2) for lipoprotein lipase (LPL) mediated TG hydrolysis. Design: Within-participant cross-over study. Setting: A University metabolic investigation unit. Participants: Ten overweight/obese men. Interventions: Participants undertook two oral fat tolerance tests, separated by 7–14 days, in which they had blood taken fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal oxygen uptake (EX); no exercise was performed before the control test (CON). Main outcome measures: Circulating TG-rich lipoprotein concentrations; affinity of chylomicrons, VLDL1, VLDL2 for LPL-mediated TG hydrolysis. Results: Exercise significantly reduced fasting VLDL1-TG concentration (CON: 0.49(0.33–0.72) mmol.l−1, EX: 0.36(0.22–0.59) mmol.l−1, [geometric means (95% confidence interval)]; p=0.04). Time-averaged postprandial chylomicron-TG (CON: 0.55±0.10 mmol.l−1, EX: 0.39±0.08 mmol.l−1, [mean±SEM], p=0.03) and VLDL1-TG (CON: 0.85±0.13 mmol.l−1, EX: 0.66±0.10 mmol.l−1, p=0.01) concentrations were both lower in EX than CON. Affinity of VLDL1 for LPL-mediated TG hydrolysis increased by 2.2(1.3–3.7) fold (geometric mean (95% confidence interval)) (p=0.02) in the fasted state and 2.6(1.8–2.6) fold (p=0.001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials. Conclusions: Exercise increases affinity of VLDL1 for LPL-mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to exercise's TG-lowering effect.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gill, Professor Jason and Bedford, Mrs Dorothy and Caslake, Professor Muriel and Cooney, Mrs Josephine and GHAFOURI, Khloud and Wilson, Mr John
Authors: GHAFOURI, K., Cooney, J., Bedford, D. K., Wilson, J., Caslake, M. J., and Gill, J. M.R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of Clinical Endocrinology and Metabolism
Publisher:The Endocrine Society
ISSN:0021-972X
ISSN (Online):1945-7197
Copyright Holders:Copyright © 2015 The Endocrine Society
First Published:First published in Journal of Clinical Endocrinology and Metabolism
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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