Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells

Kranc, K. R. et al. (2015) Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells. Stem Cells, 33(3), pp. 699-712. (doi:10.1002/stem.1889) (PMID:25377420)

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Abstract

Identifying novel players of the pluripotency gene regulatory network centered on Oct4, Sox2, and Nanog as well as delineating the interactions within the complex network is key to understanding self-renewal and early cell fate commitment of embryonic stem cells (ESC). While overexpression of the transcriptional regulator Cited2 sustains ESC pluripotency, its role in ESC functions remains unclear. Here, we show that Cited2 is important for proliferation, survival, and self-renewal of mouse ESC. We position Cited2 within the pluripotency gene regulatory network by defining Nanog, Tbx3, and Klf4 as its direct targets. We also demonstrate that the defects caused by Cited2 depletion are, at least in part, rescued by Nanog constitutive expression. Finally, we demonstrate that Cited2 is required for and enhances reprogramming of mouse embryonic fibroblasts to induced pluripotent stem cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Subramani, Miss Chithra and Armesilla-Diaz, Dr Alejandro and Wheadon, Dr Helen and Kranc, Professor Kamil
Authors: Kranc, K. R., Oliveira, D. V., Armesilla-Diaz, A., Pacheco-Leyva, I., Catarina Matias, A., Luisa Escapa, A., Subramani, C., Wheadon, H., Trindade, M., Nichols, J., Kaji, K., Enver, T., and Bragança, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Stem Cells
Publisher:AlphaMed Press, Inc.
ISSN:1066-5099
ISSN (Online):1549-4918

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