In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity

Derakhshan, M. H. et al. (2015) In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity. Gut, 2015(64), pp. 1705-1714. (doi: 10.1136/gutjnl-2014-308914) (PMID:25753030)

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Abstract

Introduction: Recently, we showed that the length of cardiac mucosa in healthy volunteers correlated with age and obesity. We have now examined the immunohistological characteristics of this expanded cardia to determine whether it may be due to columnar metaplasia of the distal oesophagus. Methods: We used the squamocolumnar junction (SCJ), antral and body biopsies from the 52 Helicobacter pylori-negative healthy volunteers who had participated in our earlier physiological study and did not have hiatus hernia, transsphincteric acid reflux, Barrett's oesophagus or intestinal metaplasia (IM) at cardia. The densities of inflammatory cells and reactive atypia were scored at squamous, cardiac and oxyntocardiac mucosa of SCJ, antrum and body. Slides were stained for caudal type homeobox 2 (CDX-2), villin, trefoil factor family 3 (TFF-3) and liver–intestine (LI)-cadherin, mucin MUC1, Muc-2 and Muc-5ac. In addition, biopsies from 15 Barrett's patients with/without IM were stained and scored as comparison. Immunohistological characteristics were correlated with parameters of obesity and high-resolution pH metry recording. Results: Cardiac mucosa had a similar intensity of inflammatory infiltrate to non-IM Barrett's and greater than any of the other upper GI mucosae. The immunostaining pattern of cardiac mucosa most closely resembled non-IM Barrett's showing only slightly weaker CDX-2 immunostaining. In distal oesophageal squamous mucosa, expression of markers of columnar differentiation (TFF-3 and LI-cadherin) was apparent and these correlated with central obesity (correlation coefficient (CC)=0.604, p=0.001 and CC=0.462, p=0.002, respectively). In addition, expression of TFF-3 in distal oesophageal squamous mucosa correlated with proximal extension of gastric acidity within the region of the lower oesophageal sphincter (CC=−0.538, p=0.001). Conclusions: These findings are consistent with expansion of cardia in healthy volunteers occurring by squamo columnar metaplasia of distal oesophagus and aggravated by central obesity. This metaplastic origin of expanded cardia may be relevant to the substantial proportion of cardia adenocarcinomas unattributable to H. pylori or transsphincteric acid reflux.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Orange, Miss Clare and Robertson, Dr Elaine and Ferrier, Mr Roderick and Harvey, Mr Tim and McColl, Professor Kenneth and Going, Dr James and Wirz, Sister Angela and Derakhshan, Dr Mohammad and Ballantyne, Dr Stuart and Lee, Dr Yeong
Authors: Derakhshan, M. H., Robertson, E. V., Lee, Y. Y., Harvey, T., Ferrier, R. K., Wirz, A. A., Orange, C., Ballantyne, S. A., Hanvey, S. L., Going, J. J., and McColl, K. E.L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Gut
Publisher:BMJ Publishing Group Ltd & British Society of Gastroenterology
ISSN:0017-5749
ISSN (Online):1468-3288

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
510251Does intra-abdominal fat accumulation induce intrasphincteric acid reflux and metaplasia at gastro-oesophageal junction?Kenneth MccollScottish Executive Health Department (SEHHD-CSO)CZB/4/709RI CARDIOVASCULAR & MEDICAL SCIENCES