Urinary proteomic biomarkers to predict cardiovascular events

Brown, C. E. et al. (2015) Urinary proteomic biomarkers to predict cardiovascular events. Proteomics Clinical Applications, 9(5-6), pp. 610-617. (doi: 10.1002/prca.201400195) (PMID:25786980)

104348.pdf - Accepted Version


Publisher's URL: http://dx.doi.org/10.1002/prca.201400195


Purpose: We have previously demonstrated associations between the urinary proteome profile and coronary artery disease (CAD) in cross-sectional studies. Here we evaluate the potential of a urinary proteomic panel as a predictor of CAD in the Hypertensive Atherosclerotic Cardiovascular Disease (HACVD) sub-study population of the ASCOT study.<p></p> Experimental design: Thirty-seven cases with primary CAD endpoint were matched for sex and age to controls who had not reached a CAD endpoint during the study. Spot urine samples were analysed using capillary electrophoresis (CE) coupled to Micro-TOF mass spectrometry (MS). A previously developed 238-marker CE-MS model for diagnosis of CAD (CAD238) was assessed for its predictive potential.<p></p> Results: Sixty urine samples (32 cases; 28 controls; 88% male, mean age 64±5 years) were analysed. There was a trend towards healthier values in controls for the CAD model classifier (-0.432±0.326 vs -0.587±0.297, P = 0.170), and the CAD model showed statistical significance on Kaplan-Meier survival analysis P = 0.021. We found 190 individual markers out of 1501 urinary peptides that separated cases and controls (AUC>0.6). Of these, 25 peptides were also components of CAD238.<p></p> Conclusion & clinical relevance: A urinary proteome panel originally developed in a cross-sectional study predicts CAD endpoints independent of age and sex in a well controlled prospective study.<p></p>

Item Type:Articles
Glasgow Author(s) Enlighten ID:Mischak, Professor Harald and Mullen, Dr Bill and Dominiczak, Professor Anna and Delles, Professor Christian and Sattar, Professor Naveed and Stalmach, Dr Angelique and Brown, Dr Catriona
Authors: Brown, C. E., McCarthy, N. S., Hughes, A. D.,, Sever, P., Stalmach, A., Mullen, W., Dominiczak, A. F., Sattar, N., Mischak, H., Thom, S., Mayet, J., Stanton, A. V., and Delles, C.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
Journal Name:Proteomics Clinical Applications
ISSN (Online):1862-8354
Copyright Holders:Copyright © 2015 Wiley
First Published:First published in Proteomics Clinical Applications
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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