Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence

Tilston-Lunel, N. L., Hughes, J. , Olszanski Acrani, G., da Silva, D. E.A., Azevedo, R. S.S., Rodrigues, S. G., Vasconcelos, P. F.C., Nunes, M. R.T. and Elliott, R. M. (2015) Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence. Journal of General Virology, 96(7), pp. 1636-1650. (doi:10.1099/vir.0.000108) (PMID:25735305) (PMCID:PMC4635451)

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Abstract

Oropouche virus (OROV) is a public health threat in South America, and in particular Northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches we have determined complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil in 2009. We also report complete genome sequences of two OROV reassortants isolated from two marmosets in Minas Gerais state, southeast Brazil in 2012 that contain a novel M genome segment. Interestingly, all ten isolates posses a 947 nucleotide long S segment that lacks 11 residues in the S segment 3' UTR compared to the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated and the identification of yet another reassortant highlights the importance of bunyavirus surveillance in South America.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Olszanski Acrani, Mr Gustavo and Hughes, Dr Joseph and Elliott, Professor Richard
Authors: Tilston-Lunel, N. L., Hughes, J., Olszanski Acrani, G., da Silva, D. E.A., Azevedo, R. S.S., Rodrigues, S. G., Vasconcelos, P. F.C., Nunes, M. R.T., and Elliott, R. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Journal of General Virology 96(7):1636-1650
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
635361Molecular analyses of arbovirus-host interactionsRichard ElliottWellcome Trust (WELLCOME)099220/B/12/ZMVLS III - CENTRE FOR VIRUS RESEARCH