Tilston-Lunel, N. L., Hughes, J. , Olszanski Acrani, G., da Silva, D. E.A., Azevedo, R. S.S., Rodrigues, S. G., Vasconcelos, P. F.C., Nunes, M. R.T. and Elliott, R. M. (2015) Genetic analysis of members of the species Oropouche virus and identification of a novel M segment sequence. Journal of General Virology, 96(7), pp. 1636-1650. (doi: 10.1099/vir.0.000108) (PMID:25735305) (PMCID:PMC4635451)
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Abstract
Oropouche virus (OROV) is a public health threat in South America, and in particular Northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches we have determined complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil in 2009. We also report complete genome sequences of two OROV reassortants isolated from two marmosets in Minas Gerais state, southeast Brazil in 2012 that contain a novel M genome segment. Interestingly, all ten isolates posses a 947 nucleotide long S segment that lacks 11 residues in the S segment 3' UTR compared to the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated and the identification of yet another reassortant highlights the importance of bunyavirus surveillance in South America.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Olszanski Acrani, Mr Gustavo and Elliott, Professor Richard and Hughes, Dr Joseph |
| Authors: | Tilston-Lunel, N. L., Hughes, J., Olszanski Acrani, G., da Silva, D. E.A., Azevedo, R. S.S., Rodrigues, S. G., Vasconcelos, P. F.C., Nunes, M. R.T., and Elliott, R. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of General Virology |
| Publisher: | Society for General Microbiology |
| ISSN: | 0022-1317 |
| ISSN (Online): | 1465-2099 |
| Copyright Holders: | Copyright © 2015 The Authors |
| First Published: | First published in Journal of General Virology 96(7):1636-1650 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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