Zhang, P., Ren, L., Zhang, X., Shan, Y., Wang, Y., Ji, Y., Yin, H. , Huang, W. E., Xu, J. and Ma, B. (2015) Raman-activated cell sorting based on dielectrophoretic single-cell trap and release. Analytical Chemistry, 87(4), pp. 2282-2289. (doi: 10.1021/ac503974e) (PMID:25607599)
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Abstract
Raman-activated cell sorting (RACS) is a promising single-cell technology that holds several significant advantages, as RACS is label-free, information-rich, and potentially in situ. To date, the ability of the technique to identify single cells in a high-speed flow has been limited by inherent weakness of the spontaneous Raman signal. Here we present an alternative pause-and-sort RACS microfluidic system that combines positive dielectrophoresis (pDEP) for single-cell trap and release with a solenoid-valve-suction-based switch for cell separation. This has allowed the integration of trapping, Raman identification, and automatic separation of individual cells in a high-speed flow. By exerting a periodical pDEP field, single cells were trapped, ordered, and positioned individually to the detection point for Raman measurement. As a proof-of-concept demonstration, a mixture of two cell strains containing carotenoid-producing yeast (9%) and non-carotenoid-producing Saccharomyces cerevisiae (91%) was sorted, which enriched the former to 73% on average and showed a fast Raman-activated cell sorting at the subsecond level.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing |
Authors: | Zhang, P., Ren, L., Zhang, X., Shan, Y., Wang, Y., Ji, Y., Yin, H., Huang, W. E., Xu, J., and Ma, B. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Analytical Chemistry |
Journal Abbr.: | Anal. chem. |
ISSN: | 0003-2700 |
ISSN (Online): | 1520-6882 |
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