Effect of flunixin meglumine and firocoxib on ex vivo cyclooxygenase activity in horses undergoing surgery

Duz, M., Parkin, T. , Cullander, R. M. and Marshall, J. (2015) Effect of flunixin meglumine and firocoxib on ex vivo cyclooxygenase activity in horses undergoing surgery. American Journal of Veterinary Research, 76(3), pp. 208-215. (doi:10.2460/ajvr.76.3.208) (PMID:25710756)

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Abstract

OBJECTIVE: To evaluate ex vivo cyclooxygenase (COX) inhibition and compare in vitro and ex vivo COX-1 inhibition by flunixin meglumine and firocoxib in horses.<p></p> ANIMALS: 4 healthy horses for in vitro experiments and 12 healthy horses (6 males and 6 females; 5 Thoroughbreds, 5 Warmbloods, and 2 ponies) undergoing elective surgery for ex vivo experiments.<p></p> PROCEDURES: 12 horses received flunixin meglumine (1.1 mg/kg, IV, q 12 h) or firocoxib (0.09 mg/kg, IV, q 24 h). Blood samples were collected before (baseline) and 2 and 24 hours after NSAID administration. Prostanoids (thromboxane B2, prostaglandin E2, and prostaglandin E metabolites) served as indicators of COX activity, and serum drug concentrations were measured by use of high-performance liquid chromatography. An in vitro coagulation-induced thromboxane B2 assay was used to calculate drug concentration-COX-1 inhibition curves. Effect of time and treatment on COX activity was determined. Agreement between in vitro and ex vivo measurement of COX activity was assessed with Bland-Altman analysis.<p></p> RESULTS: At 2 and 24 hours after NSAID administration, COX-1 activity was reduced, compared with baseline activity, for the flunixin meglumine group only and relative COX-1 activity was significantly greater for the firocoxib group, compared with that for the flunixin meglumine group. There was no significant change in COX-2 activity after surgery for either group. Bland-Altman analysis revealed poor agreement between in vitro and ex vivo measurement of COX-1 activity.<p></p> CONCLUSIONS AND CLINICAL RELEVANCE: Compared with flunixin meglumine, firocoxib had COX-1-sparing effects ex vivo in equine patients that underwent elective surgery.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Parkin, Professor Timothy and Marshall, Dr John
Authors: Duz, M., Parkin, T., Cullander, R. M., and Marshall, J.
Subjects:S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:American Journal of Veterinary Research
Journal Abbr.:AJVR
Publisher:American Veterinary Medical Association
ISSN:0002-9645
ISSN (Online):1943-5681

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