Cloning of F DNA fragments carrying the origin of transfer oriT and the fertility inhibition gene finP

Johnson, D., Everett, R. and Willetts, N. (1981) Cloning of F DNA fragments carrying the origin of transfer oriT and the fertility inhibition gene finP. Journal of Molecular Biology, 153(2), pp. 187-202. (doi:10.1016/0022-2836(81)90273-4) (PMID:6279869)

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Abstract

The two SalI fragments derived from the F transfer region that are bounded at one end by the SalI cleavage site in traM were cloned into pBR322. From these, smaller (540 bases) SalI-BglII fragments were subcloned to give plasmids containing the origin of transfer oriT (pED806) and finP (pED812), respectively, but no entire tra genes. All four plasmids were characterized by genetic tests and by restriction endonuclease analysis. pED806 could not be used to search for an F oriT-related “relaxation complex” because of its unexpected instability in the presence of Flac, and extensive efforts to prepare such a complex using other oriT+ plasmids were unsuccessful. We therefore suggest that a cell-free F relaxation complex does not exist. Protein synthesis directed by pED812 in minicells allowed the finP product to be tentatively identified as a 4000 Mr, protein.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Everett, Professor Roger
Authors: Johnson, D., Everett, R., and Willetts, N.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Molecular Biology
Publisher:Academic Press
ISSN:0022-2836
ISSN (Online):0022-2836

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