Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking

Sadler, J. B.A., Bryant, N. J. and Gould, G. W. (2015) Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking. Molecular Biology of the Cell, 26(3), pp. 530-536. (doi:10.1091/mbc.E14-09-1368) (PMID:25501368)

Full text not currently available from Enlighten.


The fusion of GLUT4-containing vesicles with the plasma membrane of adipocytes is a key facet of insulin action. This process is mediated by the formation of functional soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) complexes between the plasma membrane t-SNARE complex and the vesicle v-SNARE or VAMP. The t-SNARE complex consists of Syntaxin4 and SNAP23, and whereas many studies identify VAMP2 as the v-SNARE, others suggest that either VAMP3 or VAMP8 may also fulfil this role. Here we characterized the levels of expression, distribution, and association of all the VAMPs expressed in 3T3-L1 adipocytes to provide the first systematic analysis of all members of this protein family for any cell type. Despite our finding that all VAMP isoforms form SDS-resistant SNARE complexes with Syntaxin4/SNAP23 in vitro, a combination of levels of expression (which vary by >30-fold), subcellular distribution, and coimmunoprecipitation analyses lead us to propose that VAMP2 is the major v-SNARE involved in GLUT4 trafficking to the surface of 3T3-L1 adipocytes.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Sadler, Miss Jessica and Gould, Professor Gwyn and Bryant, Dr Nia
Authors: Sadler, J. B.A., Bryant, N. J., and Gould, G. W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Molecular Biology of the Cell
Publisher:American Society for Cell Biology
ISSN (Online):1939-4586

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
580731How does tyrosine phosphorylation of SNARE proteins control GLUT4 vesicle fusion with the plasma membrane?Gwyn GouldDiabetes UK (DIABETESUK)11/0004289RI MOLECULAR CELL & SYSTEMS BIOLOGY